BMRB Entry 50516

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50516

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Title:
Nsp3c SUD-M
Deposition date:
2020-10-19
Original release date:
2021-01-25
Authors:
Gallo, Angelo; Tsika, Aikaterini; Fourkiotis, Nikolaos; Spyroulias, Georgios
Citation:

Citation: Gallo, Angelo; Tsika, Aikaterini; Fourkiotis, Nikolaos; Cantini, Francesca; Banci, Lucia; Sreeramulu, Sridhar; Schwalbe, Harald; Spyroulias, Georgios. "1H,13C and 15N chemical shift assignments of the SUD domains of SARS-CoV-2 non-structural protein 3c: The SUD-M and SUD-C domains"  Biomol. NMR Assign. 15, 165-171 (2021).
PubMed: 33423172

Assembly members:

Assembly members:
entity_1, polymer, 129 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: SARS-CoV-2   Taxonomy ID: 2697049   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betacoronavirus HCoV-SARS

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSHMGTVSWNLREMLAHAEE TRKLMPVCVETKAIVSTIQR KYKGIKIQEGVVDYGARFYF YTSKTTVASLINTLNDLNET LVTMPLGYVTHGLNLEEAAR YMRSLKVPATVSVSSPDAVT AYNGYLTSS

Data sets:
Data typeCount
13C chemical shifts521
15N chemical shifts121
1H chemical shifts829

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Nsp3c_SUD_M1

Entities:

Entity 1, Nsp3c_SUD_M 129 residues - Formula weight is not available

1   GLYSERHISMETGLYTHRVALSERTRPASN
2   LEUARGGLUMETLEUALAHISALAGLUGLU
3   THRARGLYSLEUMETPROVALCYSVALGLU
4   THRLYSALAILEVALSERTHRILEGLNARG
5   LYSTYRLYSGLYILELYSILEGLNGLUGLY
6   VALVALASPTYRGLYALAARGPHETYRPHE
7   TYRTHRSERLYSTHRTHRVALALASERLEU
8   ILEASNTHRLEUASNASPLEUASNGLUTHR
9   LEUVALTHRMETPROLEUGLYTYRVALTHR
10   HISGLYLEUASNLEUGLUGLUALAALAARG
11   TYRMETARGSERLEULYSVALPROALATHR
12   VALSERVALSERSERPROASPALAVALTHR
13   ALATYRASNGLYTYRLEUTHRSERSER

Samples:

sample_1: entity_1, [U-13C; U-15N], 0.9 mM; NaPi 50 mM; NaCl 50 mM; DTT 2 mM; EDTA 2 mM

sample_conditions_1: ionic strength: 104 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
(H)CCH-TOCSY 3Dsample_1isotropicsample_conditions_1
HBHA(CBCACO)NHsample_1isotropicsample_conditions_1
2D HSQC HNsample_1isotropicsample_conditions_1
1D 1Hsample_1isotropicsample_conditions_1
2D HSQC HNsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
2D HSQC HNsample_1isotropicsample_conditions_1
1D 1Hsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
1D 1Hsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
1D 1Hsample_1isotropicsample_conditions_1
1D 1Hsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
2D HSQC HNsample_1isotropicsample_conditions_1
2D HSQC HNsample_1isotropicsample_conditions_1
2D HSQC HNsample_1isotropicsample_conditions_1
1D 1Hsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
2D HSQC HNsample_1isotropicsample_conditions_1
2D HSQC HNsample_1isotropicsample_conditions_1
1D 1Hsample_1isotropicsample_conditions_1
2D HSQC HNsample_1isotropicsample_conditions_1
2D HSQC HNsample_1isotropicsample_conditions_1
1D 1Hsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

LOGS v2.2 - collection

CARA - chemical shift assignment, peak picking

TOPSPIN v4.0.6 - collection

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks