BMRB Entry 50509

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50509

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Title:
Backbone and sidechain assignments of RRM1 domain of human RBM6
Deposition date:
2020-10-12
Original release date:
2020-10-16
Authors:
Aggarwal, Priyanka; Bhavesh, Neel
Citation:

Citation: Aggarwal, Priyanka; Bhavesh, Neel. "1H, 13C, 15N chemical shift assignments for RRM1 domain of human RBM6"  The BMRB entry is the only known published source for the data..

Assembly members:

Assembly members:
entity_1, polymer, 87 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAMGHMSRLIRLSGVPEDAT KEEILNAFRTPDGMPVKNLQ LKEYNTGYDYGYVCVEFSLL EDAIGCMEANQGTLMIQDKE VTLEYVS

Data sets:
Data typeCount
13C chemical shifts311
15N chemical shifts73
1H chemical shifts448

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RRM1 domain of human RBM61

Entities:

Entity 1, RRM1 domain of human RBM6 87 residues - Formula weight is not available

1   GLYALAMETGLYHISMETSERARGLEUILE
2   ARGLEUSERGLYVALPROGLUASPALATHR
3   LYSGLUGLUILELEUASNALAPHEARGTHR
4   PROASPGLYMETPROVALLYSASNLEUGLN
5   LEULYSGLUTYRASNTHRGLYTYRASPTYR
6   GLYTYRVALCYSVALGLUPHESERLEULEU
7   GLUASPALAILEGLYCYSMETGLUALAASN
8   GLNGLYTHRLEUMETILEGLNASPLYSGLU
9   VALTHRLEUGLUTYRVALSER

Samples:

sample_1: RBM6 RRM1 domain, [U-99% 13C; U-99% 15N], 1 ± 0.01 mM; Sodium Phosphate 50 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCACONHsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.5pl6 - collection

CARA - data analysis

TOPSPIN v4.0.9 - data processing

NMR spectrometers:

  • Bruker AVANCE III 500.15 MHz

Related Database Links:

UNP P78332
AlphaFold Q86SS3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks