BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 50496

Title: Sarcomeric intrinsically disordered protein FATZ-1 (N-FATZ-1)   PubMed: 34049882

Deposition date: 2020-10-02 Original release date: 2021-05-31

Authors: Zawadzka-Kazimierczuk, Anna; Sponga, Antonio; Mateos, Borja; Mlynek, Georg; Arolas, Joan; Schwarz, Thomas; Djinovic-Carugo, Kristina; Kozminski, Wiktor; Konrat, Robert

Citation: Sponga, Antonio; Arolas, Joan; Schwarz, Thomas; Jeffries, Cy; Rodriguez Chamorro, Ariadna; Kostan, Julius; Ghisleni, Andrea; Drepper, Friedel; Polyansky, Anton; De Almeida Ribeiro, Euripedes; Pedron, Miriam; Zawadzka-Kazimierczuk, Anna; Mlynek, Georg; Peterbauer, Thomas; Doto, Pierantonio; Schreiner, Claudia; Hollerl, Eneda; Mateos, Borja; Geist, Leonhard; Faulkner, Georgine; Kozminski, Wiktor; Svergun, Dmitri; Warscheid, Bettina; Zagrovic, Bojan; Gautel, Mathias; Konrat, Robert; Djinovic-Carugo, Kristina. "Order from disorder in the sarcomere: FATZ forms a fuzzy but tight complex and phase-separated condensates with alpha-actinin."  Sci. Adv. 7, eabg7653-eabg7653 (2021).

Assembly members:
entity_1, polymer, 191 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-46

Entity Sequences (FASTA):
entity_1: MAHHHHHHVDDDDKIMPLSG TPAPNKKRKSSKLIMELTGG GQESSGLNLGKKISVPRDVM LEELSLLTNRGSKMFKLRQM RVEKFIYENHPDVFSDSSMD HFQKFLPTVGGQLGTAGQGF SYSKSNGRGGSQAGGSGSAG QYGSDQQHHLGSGSGAGGTG GPAGQAGRGGAAGTAGVGET GSGDQAGGEAE

Data sets:
Data typeCount
13C chemical shifts357
15N chemical shifts150
1H chemical shifts466

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N-FATZ-11

Entities:

Entity 1, N-FATZ-1 191 residues - Formula weight is not available

1   METALAHISHISHISHISHISHISVALASP
2   ASPASPASPLYSILEMETPROLEUSERGLY
3   THRPROALAPROASNLYSLYSARGLYSSER
4   SERLYSLEUILEMETGLULEUTHRGLYGLY
5   GLYGLNGLUSERSERGLYLEUASNLEUGLY
6   LYSLYSILESERVALPROARGASPVALMET
7   LEUGLUGLULEUSERLEULEUTHRASNARG
8   GLYSERLYSMETPHELYSLEUARGGLNMET
9   ARGVALGLULYSPHEILETYRGLUASNHIS
10   PROASPVALPHESERASPSERSERMETASP
11   HISPHEGLNLYSPHELEUPROTHRVALGLY
12   GLYGLNLEUGLYTHRALAGLYGLNGLYPHE
13   SERTYRSERLYSSERASNGLYARGGLYGLY
14   SERGLNALAGLYGLYSERGLYSERALAGLY
15   GLNTYRGLYSERASPGLNGLNHISHISLEU
16   GLYSERGLYSERGLYALAGLYGLYTHRGLY
17   GLYPROALAGLYGLNALAGLYARGGLYGLY
18   ALAALAGLYTHRALAGLYVALGLYGLUTHR
19   GLYSERGLYASPGLNALAGLYGLYGLUALA
20   GLU

Samples:

sample_1: N-FATZ-1, [U-99% 13C; U-99% 15N], 1.2 mM; TRIS 50 mM; NaCl 150 mM; EDTA 1 mM; Arginine 500 mM

sample_conditions_1: ionic strength: 0.7 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
5D HN(CA)CONHsample_1isotropicsample_conditions_1
5D (HACA)CON(CA)CONHsample_1isotropicsample_conditions_1
5D (HACA)CON(CO)CONHsample_1isotropicsample_conditions_1
5D HabCabCONHsample_1isotropicsample_conditions_1

Software:

VNMRj - experiments set up and acquisition

TSAR - resonance assignment

SPARKY - spectra display, peak picking

NMR spectrometers:

  • Agilent DD2 600 MHz

Related Database Links:

UNP Q9NP98

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts