BMRB Entry 50485

Title:
Mutant of human thioreodoxin 1 (C32S/C35S/C69S/C73S)
Deposition date:
2020-09-28
Original release date:
2021-04-13
Authors:
Almeida, Vitor; Almeida, Fabio
Citation:

Citation: Almeida, Vitor; Iqbal, Anwar; Almeida, Fabio. "1H, 15N and 13C backbone and side-chain assignments of reduced and S-nitrosated C62only mutant of human thioredoxin"  Biomol. NMR Assignments 15, 261-265 (2021).
PubMed: 33751377

Assembly members:

Assembly members:
entity_1, polymer, 105 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3a

Data sets:
Data typeCount
13C chemical shifts462
15N chemical shifts109
1H chemical shifts733

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C62only1

Entities:

Entity 1, C62only 105 residues - Formula weight is not available

1   METVALLYSGLNILEGLUSERLYSTHRALA
2   PHEGLNGLUALALEUASPALAALAGLYASP
3   LYSLEUVALVALVALASPPHESERALATHR
4   TRPSERGLYPROSERLYSMETILELYSPRO
5   PHEPHEHISSERLEUSERGLULYSTYRSER
6   ASNVALILEPHELEUGLUVALASPVALASP
7   ASPCYSGLNASPVALALASERGLUSERGLU
8   VALLYSSERMETPROTHRPHEGLNPHEPHE
9   LYSLYSGLYGLNLYSVALGLYGLUPHESER
10   GLYALAASNLYSGLULYSLEUGLUALATHR
11   ILEASNGLULEUVAL

Samples:

sample_1: C62only mutant, [U-99% 13C; U-99% 15N], 400 uM; NaCl 50 mM; Phosphate Buffer 30 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

CcpNMR - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 800 MHz
  • Bruker AVANCE III 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks