BMRB Entry 50480

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50480

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Title:
1H, 13C, and 15N resonance assignment for human Atg3 with deletion of 90 to 190 residues in bicelles
Deposition date:
2020-09-23
Original release date:
2020-11-25
Authors:
Tian, Yansheng; Tyndall, Erin; Tian, Fang
Citation:

Citation: Ye, Yansheng; Tyndall, Erin; Bui, Van; Tang, Zhenyuan; Shen, Yan; Jiang, Xuejun; Flanagan, John; Wang, Hong-Gang; Tian, Fang. "An N-terminal conserved region in human Atg3 couples membrane curvature sensitivity to conjugase activity during autophagy"  Nat. Commun. 12, 374-374 (2021).
PubMed: 33446636

Assembly members:

Assembly members:
entity_1, polymer, 213 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MQNVINTVKGKALEVAEYLT PVLKESKFKETGVITPEEFV AAGDHLVHHCPTWQWATGEE LKVKAYLPTGKQFLVTKNVP CYKRCKQMEEDAILQTRTYD LYITYDKYYQTPRLWLFGYD EQRQPLTVEHMYEDISQDHV KKTVTIENHPHLPPPPMCSV HPCRHAEVMKKIIETVAEGG GELGVHMYLLIFLKFVQAVI PTIEYDYTRHFTM

Data sets:
Data typeCount
13C chemical shifts486
15N chemical shifts155
1H chemical shifts155

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1human Atg3 with deletion of 90 to 190 residues1

Entities:

Entity 1, human Atg3 with deletion of 90 to 190 residues 213 residues - Formula weight is not available

1   METGLNASNVALILEASNTHRVALLYSGLY
2   LYSALALEUGLUVALALAGLUTYRLEUTHR
3   PROVALLEULYSGLUSERLYSPHELYSGLU
4   THRGLYVALILETHRPROGLUGLUPHEVAL
5   ALAALAGLYASPHISLEUVALHISHISCYS
6   PROTHRTRPGLNTRPALATHRGLYGLUGLU
7   LEULYSVALLYSALATYRLEUPROTHRGLY
8   LYSGLNPHELEUVALTHRLYSASNVALPRO
9   CYSTYRLYSARGCYSLYSGLNMETGLUGLU
10   ASPALAILELEUGLNTHRARGTHRTYRASP
11   LEUTYRILETHRTYRASPLYSTYRTYRGLN
12   THRPROARGLEUTRPLEUPHEGLYTYRASP
13   GLUGLNARGGLNPROLEUTHRVALGLUHIS
14   METTYRGLUASPILESERGLNASPHISVAL
15   LYSLYSTHRVALTHRILEGLUASNHISPRO
16   HISLEUPROPROPROPROMETCYSSERVAL
17   HISPROCYSARGHISALAGLUVALMETLYS
18   LYSILEILEGLUTHRVALALAGLUGLYGLY
19   GLYGLULEUGLYVALHISMETTYRLEULEU
20   ILEPHELEULYSPHEVALGLNALAVALILE
21   PROTHRILEGLUTYRASPTYRTHRARGHIS
22   PHETHRMET

Samples:

sample_1: human Atg3 with deletion of 90 to 190 residues in bicelles, [U-13C; U-15N; U-2H], 0.5 mM; sodium chloride 150 mM; HEPES 50 mM; TCEP 2 mM; DMPC 25 mM; DHPC 200 mM; DMPG 10 mM; LPE 15 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1

Software:

NMRPipe, NMRView - data processing and analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks