BMRB Entry 50467

Title:
Backbone 1H, 13C and 15N Chemical Shift Assignments for Relaxed State of S65-phosphorylated NEDD8
Deposition date:
2020-09-12
Original release date:
2021-08-24
Authors:
Stuber, Katrin; Schneider, Tobias; Werner, Jill; Kovermann, Michael; Marx, Andreas; Scheffner, Martin
Citation:

Citation: Stuber, Katrin; Schneider, Tobias; Werner, Jill; Kovermann, Michael; Marx, Andreas; Scheffner, Martin. "Structural and functional consequences of NEDD8 phosphorylation"  Nat. Commun. 12, 5939-5939 (2021).
PubMed: 34642328

Assembly members:

Assembly members:
entity_1, polymer, 76 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pKS NEDD8 S65TAG-His6

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts131
15N chemical shifts63
1H chemical shifts63

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1pNEDD81

Entities:

Entity 1, pNEDD8 76 residues - Formula weight is not available

1   METLEUILELYSVALLYSTHRLEUTHRGLY
2   LYSGLUILEGLUILEASPILEGLUPROTHR
3   ASPLYSVALGLUARGILELYSGLUARGVAL
4   GLUGLULYSGLUGLYILEPROPROGLNGLN
5   GLNARGLEUILETYRSERGLYLYSGLNMET
6   ASNASPGLULYSTHRALAALAASPTYRLYS
7   ILELEUGLYGLYSEPVALLEUHISLEUVAL
8   LEUALALEUARGGLYGLY

Samples:

sample_1: pNEDD8, [U-15N], 80 uM; HEPES 20 mM; sodium chloride 150 mM

sample_2: pNEDD8, [U-13C; U-15N], 320 uM; HEPES 20 mM; sodium chloride 150 mM

sample_conditions_1: pH: 7.4; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N TROSY-HSQCsample_2isotropicsample_conditions_1
3D TROSY-HNCAsample_2isotropicsample_conditions_1
3D TROSY-HNCOsample_2isotropicsample_conditions_1
3D TROSY-HN(CO)CACBsample_2isotropicsample_conditions_1
3D TROSY-HNCACBsample_2isotropicsample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

NMRView - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks