BMRB Entry 50448

Title:
1H-13C-15N Sars Unique Domain CoV-2 (nsp3c) N-terminal domain   PubMed: 33225414
Deposition date:
2020-08-31
Original release date:
2020-12-22
Authors:
Gallo, Angelo; Tsika, Aikaterini; Fourkiotis, Nikos; Spyroulias, Georgios
Citation:

Citation: Gallo, Angelo; Tsika, Aikaterini; Fourkiotis, Nikolaos; Cantini, Francesca; Banci, Lucia; Sreeramulu, Sridhar; Schwalbe, Harald; Spyroulias, Georgios. "1H, 13C and 15N chemical shift assignments of the SUD domains of SARS-CoV-2 non-structural protein 3c: "the N-terminal domain-SUD-N""  Biomol. NMR Assign. 15, 85-89 (2021).

Assembly members:

Assembly members:
entity_1, polymer, 142 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: SARS-CoV-2   Taxonomy ID: 2697049   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betacoronavirus HCoV-SARS

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGex4T-1

Experimental source:

Natural source:   Common Name: SARS-CoV-2   Taxonomy ID: 2697049   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betacoronavirus HCoV-SARS

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGex4T-1

Data sets:
Data typeCount
13C chemical shifts568
15N chemical shifts127
1H chemical shifts916

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N-terminal domain1

Entities:

Entity 1, N-terminal domain 142 residues - Formula weight is not available

GS are the remaining of the cleavage site

1   GLYSERGLNASPASPLYSLYSILELYSALA
2   CYSVALGLUGLUVALTHRTHRTHRLEUGLU
3   GLUTHRLYSPHELEUTHRGLUASNLEULEU
4   LEUTYRILEASPILEASNGLYASNLEUHIS
5   PROASPSERALATHRLEUVALSERASPILE
6   ASPILETHRPHELEULYSLYSASPALAPRO
7   TYRILEVALGLYASPVALVALGLNGLUGLY
8   VALLEUTHRALAVALVALILEPROTHRLYS
9   LYSALAGLYGLYTHRTHRGLUMETLEUALA
10   LYSALALEUARGLYSVALPROTHRASPASN
11   TYRILETHRTHRTYRPROGLYGLNGLYLEU
12   ASNGLYTYRTHRVALGLUGLUALALYSTHR
13   VALLEULYSLYSCYSLYSSERALAPHETYR
14   ILELEUPROSERILEILESERASNGLULYS
15   GLNGLU

Samples:

sample_1: SARS Unique Domain N-terminal domain of SARS-CoV-2, [U-100% 13C; U-100% 15N], 0.95 mM; D2O, [U-2H], 10%; DTT 2 mM; H2O 90%; sodium azide 2 mM; EDTA 2 mM; sodium chloride 50 mM; sodium phosphate 50 mM; Protease inhibitor 1 na

sample_conditions_1: ionic strength: 100 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCANHsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v4.0.6 - collection, processing

CARA - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III HD 700 MHz

Related Database Links:

GB MT066156.1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks