BMRB Entry 50444

Title:
Amide assignments of human GMCSF at low pH
Deposition date:
2020-08-25
Original release date:
2021-04-16
Authors:
Cui, Jennifer; Lisi, George
Citation:

Citation: Cui, Jennifer; Zhang, Fuming; Nierzwicki, Lukasz; Palermo, Giulia; Linhardt, Robert; Lisi, George. "Mapping the Structural and Dynamic Determinants of pH-Sensitive Heparin Binding to Granulocyte Macrophage Colony Stimulating Factor"  Biochemistry 59, 3541-3553 (2020).
PubMed: 32897051

Assembly members:

Assembly members:
entity_1, polymer, 125 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-15b

Data sets:
Data typeCount
15N chemical shifts98
1H chemical shifts97

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GMCSF1

Entities:

Entity 1, GMCSF 125 residues - Formula weight is not available

1   ALAARGSERPROSERPROSERTHRGLNPRO
2   TRPGLUHISVALASNALAILEGLNGLUALA
3   ARGARGLEULEUASNLEUSERARGASPTHR
4   ALAALAGLUMETASNGLUTHRVALGLUVAL
5   ILESERGLUMETPHEASPLEUGLNGLUPRO
6   THRCYSLEUGLNTHRARGLEUGLULEUTYR
7   LYSGLNGLYLEUARGGLYSERLEUTHRLYS
8   LEULYSGLYPROLEUTHRMETMETALASER
9   HISTYRLYSGLNHISCYSPROPROTHRPRO
10   GLUTHRSERCYSALATHRGLNILEILETHR
11   PHEGLUSERPHELYSGLUASNLEULYSASP
12   PHELEULEUVALILEPROPHEASPCYSTRP
13   GLUPROVALGLNGLU

Samples:

sample_1: GMCSF, [U-100% 15N], 300 uM; HEPES 20 mM; EDTA 1 mM

sample_conditions_1: ionic strength: 0.02 M; pH: 5.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks