BMRB Entry 50431

Title:
ETV6 PNT Domain with an V112E PNT domain complexed to an unlabelled A93D PNT domain
Deposition date:
2020-08-11
Original release date:
2021-01-25
Authors:
Gerak, Chloe; Okon, Mark; McIntosh, Lawrence
Citation:

Citation: Gerak, Chloe; Cho, Sophia; Kolesnikov, Maxim; Okon, Mark; Sessions, Richard; Murphy, Michael; Roberge, Michel; McIntosh, Lawrence. "Biophysical characterization of the ETV6 PNT domain polymerization interfaces"  J. Biol. Chem. 296, 100284-100284 (2021).
PubMed: 33450226

Assembly members:

Assembly members:
entity_1, polymer, 83 residues, Formula weight is not available
entity_2, polymer, 83 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Data sets:
Data typeCount
15N chemical shifts74
1H chemical shifts74

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ETV6 PNT Domain V112E Complexed1
2Unlabelled ETV6 PNT domain A93D2

Entities:

Entity 1, ETV6 PNT Domain V112E Complexed 83 residues - Formula weight is not available

1   METGLUGLUASPSERILEARGLEUPROALA
2   HISLEUARGLEUGLNPROILETYRTRPSER
3   ARGASPASPVALALAGLNTRPLEULYSTRP
4   ALAGLUASNGLUPHESERLEUARGPROILE
5   ASPSERASNTHRPHEGLUMETASNGLYLYS
6   ALALEULEULEULEUTHRLYSGLUASPPHE
7   ARGTYRARGSERPROHISSERGLYASPGLU
8   LEUTYRGLULEULEUGLNHISILELEULYS
9   GLNARGLYS

Entity 2, Unlabelled ETV6 PNT domain A93D 83 residues - Formula weight is not available

1   METGLUGLUASPSERILEARGLEUPROALA
2   HISLEUARGLEUGLNPROILETYRTRPSER
3   ARGASPASPVALALAGLNTRPLEULYSTRP
4   ALAGLUASNGLUPHESERLEUARGPROILE
5   ASPSERASNTHRPHEGLUMETASNGLYLYS
6   ASPLEULEULEULEUTHRLYSGLUASPPHE
7   ARGTYRARGSERPROHISSERGLYASPVAL
8   LEUTYRGLULEULEUGLNHISILELEULYS
9   GLNARGLYS

Samples:

sample_1: MOPS 20 mM; NaCl 50 mM; EDTA 0.5 mM; ETV6 PNT Domain V112E, [U-99% 13C; U-99% 15N], 210 uM; Unlabelled ETV6 PNT domain A93D 210 uM

sample_conditions_1: ionic strength: 70 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment, peak picking

TOPSPIN - collection

NMRPipe - processing

PINE - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks