BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 50330

Title: Ubkeks

Deposition date: 2020-06-17 Original release date: 2021-05-14

Authors: Delattre, Patrick; Letourneau, Danny; Lavigne, Pierre

Citation: Delattre, Patrick; Letourneau, Danny; Lavigne, Pierre. "Structural and dynamic characterization of UbKEKS, a newly indentified ubiquitin encoded in a pseudogene"  .

Assembly members:
entity_1, polymer, 76 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28A

Entity Sequences (FASTA):
entity_1: MKIFVKTLTGKTITLEVEPS DTIENVKAKIQDEEGIPPDQ QRLIFAGKKLEDGRTLSDYS IQKESTLHLVLRLRGG

Data sets:
Data typeCount
13C chemical shifts317
15N chemical shifts75
1H chemical shifts529

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ubkeks1

Entities:

Entity 1, Ubkeks 76 residues - Formula weight is not available

1   METLYSILEPHEVALLYSTHRLEUTHRGLY
2   LYSTHRILETHRLEUGLUVALGLUPROSER
3   ASPTHRILEGLUASNVALLYSALALYSILE
4   GLNASPGLUGLUGLYILEPROPROASPGLN
5   GLNARGLEUILEPHEALAGLYLYSLYSLEU
6   GLUASPGLYARGTHRLEUSERASPTYRSER
7   ILEGLNLYSGLUSERTHRLEUHISLEUVAL
8   LEUARGLEUARGGLYGLY

Samples:

sample_1: UbKEKS, [U-100% 13C; U-100% 15N], 450 uM; D2O 10%; H2O 90%; sodium phosphate 50 mM; sodium chloride 50 mM; sodium azide 0.02%

sample_3: UbKEKS, [U-100% 13C; U-100% 15N], 450 uM; D2O 100%; sodium phosphate 50 mM; sodium chloride 50 mM; sodium azide 0.02%

sample_conditions_1: ionic strength: 100 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 100 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_2
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_2
3D 1H-13C NOESYsample_1isotropicsample_conditions_2

Software:

CcpNmr Analysis v2.5 - chemical shift assignment, peak picking

TOPSPIN v3.5 - collection, processing

ARIA v2.3 - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts