BMRB Entry 50304

Title:
EapH1
Deposition date:
2020-06-04
Original release date:
2023-02-20
Authors:
Pal, Indrani; Herrera, Alvaro; Mishra, Nitin; Prakash, Om
Citation:

Citation: Mishra, Nitin; Pal, Indrani; Herrera, Alvaro; Dubey, Abhinav; Arthanari, Haribabu; Geisbrecht, Brian; Prakash, Om. "Complete non-proline backbone resonance assignments of the S. aureus neutrophil serine protease inhibitor, EapH1"  Biomol. NMR Assign. 17, 129-134 (2023).
PubMed: 37160842

Assembly members:

Assembly members:
entity_1, polymer, 114 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Staphylococcus aureus   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: BL21(DE3)

Data sets:
Data typeCount
13C chemical shifts325
15N chemical shifts111
1H chemical shifts111

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1EapH11

Entities:

Entity 1, EapH1 114 residues - Formula weight is not available

1   GLYSERTHRASPSERASNASNGLYTYRLYS
2   GLULEUTHRMETASPGLYLYSHISTHRVAL
3   PROTYRTHRILESERVALASPGLYILETHR
4   ALALEUHISARGTHRTYRPHEVALPHEPRO
5   GLUASNLYSLYSVALLEUTYRGLNGLUILE
6   ASPSERLYSVALLYSASNGLULEUALASER
7   GLNARGGLYVALTHRTHRGLULYSILEASN
8   ASNALAGLNTHRALATHRTYRTHRLEUTHR
9   LEUASNASPGLYASNLYSLYSVALVALASN
10   LEULYSLYSASNASPASPALALYSASNSER
11   ILEASPPROSERTHRILELYSGLNILEGLN
12   ILEVALVALLYS

Samples:

sample_1: EapH1, [U-100% 13C; U-100% 15N], 800 uM

sample_conditions_1: pH: 6.6; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

CARA - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks