BMRB Entry 50276

Title:
Backbone Assignments for human BCL-XL(1-212)
Deposition date:
2020-05-15
Original release date:
2020-09-18
Authors:
Ryzhov, Pavel; Yao, Yong; Marassi, Francesca
Citation:

Citation: Ryzhov, Pavel; Tian, Ye; Yao, Yong; Bobkov, Andrey; Im, Wonpil; Marassi, Francesca. "Conformational States of the Cytoprotective Protein Bcl-xL"  Biophys. J. 119, 1324-1334 (2020).
PubMed: 32888404

Assembly members:

Assembly members:
entity_1, polymer, 233 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET21b

Data sets:
Data typeCount
13C chemical shifts293
15N chemical shifts179
1H chemical shifts179

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BCL-XL1

Entities:

Entity 1, BCL-XL 233 residues - Formula weight is not available

1   METSERGLNSERASNARGGLULEUVALVAL
2   ASPPHELEUSERTYRLYSLEUSERGLNLYS
3   GLYTYRSERTRPSERGLNPHESERASPVAL
4   GLUGLUASNARGTHRGLUALAPROGLUGLY
5   THRGLUSERGLUMETGLUTHRPROSERALA
6   ILEASNGLYASNPROSERTRPHISLEUALA
7   ASPSERPROALAVALASNGLYALATHRGLY
8   HISSERSERSERLEUASPALAARGGLUVAL
9   ILEPROMETALAALAVALLYSGLNALALEU
10   ARGGLUALAGLYASPGLUPHEGLULEUARG
11   TYRARGARGALAPHESERASPLEUTHRSER
12   GLNLEUHISILETHRPROGLYTHRALATYR
13   GLNSERPHEGLUGLNVALVALASNGLULEU
14   PHEARGASPGLYVALASNTRPGLYARGILE
15   VALALAPHEPHESERPHEGLYGLYALALEU
16   CYSVALGLUSERVALASPLYSGLUMETGLN
17   VALLEUVALSERARGILEALAALATRPMET
18   ALATHRTYRLEUASNASPHISLEUGLUPRO
19   TRPILEGLNGLUASNGLYGLYTRPASPTHR
20   PHEVALGLULEUTYRGLYASNASNALAALA
21   ALAGLUSERARGLYSGLYGLNGLUARGPHE
22   ASNARGTRPPHELEUTHRGLYMETTHRVAL
23   ALAGLYVALVALLEULEUGLYSERLEUPHE
24   SERARGLYS

Samples:

sample_1: BCL-XL, [U-100% 13C; U-100% 15N], 0.5 mM; sodium phosphate 20 mM; DTT 2 mM; EDTA 1 mM

sample_conditions_1: ionic strength: 0.025 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

NMRPipe - processing

NMRViewJ - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks