BMRB Entry 50275

Title:
Maturation of the Functional Mouse CRES Amyloid from Globular Form
Deposition date:
2020-05-15
Original release date:
2020-06-08
Authors:
Hewetson, Aveline; Khan, Nazmul; Dominguez, Matthew; Do, Hoa; Kusko, Rebecca; Borcik, Collin; Rigden, Daniel; Keegan, Ronan; Sutton, Roger; Latham, Michael; Wylie, Benjamin; Cornwall, Gail
Citation:

Citation: Hewetson, Aveline; Khan, Nazmul; Dominguez, Matthew; Do, Hoa; Kusko, Rebecca; Borcik, Collin; Rigden, Daniel; Keegan, Ronan; Sutton, Roger; Latham, Michael; Wylie, Benjamin; Cornwall, Gail. "Maturation of the functional mouse CRES amyloid from globular form"  Proc. Natl. Acad. Sci. U.S.A. 117, 16363-16372 (2020).
PubMed: 32601205

Assembly members:

Assembly members:
entity_1, polymer, 129 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-cs

Data sets:
Data typeCount
13C chemical shifts534
15N chemical shifts116

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1globular CRES1

Entities:

Entity 1, globular CRES 129 residues - Formula weight is not available

1   GLYALAMETALAHISMETVALGLYVALASP
2   GLNSERLYSASNGLUVALLYSALAGLNASN
3   TYRPHEGLYSERILEASNILESERASNALA
4   ASNVALLYSGLNALAVALTRPPHEALAMET
5   LYSGLUTYRASNLYSGLUSERGLUASPLYS
6   TYRVALPHELEUVALASPLYSILELEUHIS
7   ALALYSLEUGLNILETHRASPARGMETGLU
8   TYRGLNILEASPVALGLNILESERARGSER
9   ASNCYSLYSLYSPROLEUASNASNTHRGLU
10   ASNCYSILEPROGLNLYSLYSPROGLULEU
11   GLULYSLYSMETSERCYSSERPHELEUVAL
12   GLYALALEUPROTRPASNGLYGLUPHEASN
13   LEULEUSERLYSGLUCYSLYSASPVAL

Samples:

sample_1: CRES, [U-100% 13C; U-100% 15N], 50 mM; HEPES 50 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.4; pressure: 1 atm; temperature: 277 K

Experiments:

NameSampleSample stateSample conditions
DARRsample_1isotropicsample_conditions_1
NCACXsample_1isotropicsample_conditions_1
NCOCXsample_1isotropicsample_conditions_1
CANcoCX DARRsample_1isotropicsample_conditions_1

Software:

CcpNmr Analysis - chemical shift assignment

NMRPipe - processing

CS-Rosetta - structure solution

NMR spectrometers:

  • Varian DD2 600 MHz