BMRB Entry 50258

Name: ch-TOG 1-250
Published: 2020-12-07

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR50258

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

ch-TOG 1-250

Deposition date:

2020-05-01

Original release date:

2020-12-07

Authors:

Ryan, Veronica; Naik, Mandar; Fawzi, Nicolas

Citation:

Citation: Ryan, Veronica; Perdikari, Theodora; Naik, Mandar; Saueressig, Camillo; Lins, Jeremy; Dignon, Gregory; Mittal, Jeetain; Hart, Anne; Fawzi, Nicolas. "Tyrosine phosphorylation regulates hnRNPA2 granule protein partitioning and reduces neurodegeneration" EMBO J. 40, e105001-e105001 (2021).
PubMed: 33349959

Assembly members:

Assembly members:
entity_1, polymer, 250 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pJ411

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGDDSEWLKLPVDQKCEHKL WKARLSGYEEALKIFQKIKD EKSPEWSKFLGLIKKFVTDS NAVVQLKGLEAALVYVENAH VAGKTTGEVVSGVVSKVFNQ PKAKAKELGIEICLMYIEIE KGEAVQEELLKGLDNKNPKI IVACIETLRKALSEFGSKII LLKPIIKVLPKLFESREKAV RDEAKLIAVEIYRWIRDALR PPLQNINSVQLKELEEEWVK LPTSAPRPTRFLRSQQELEA KLEQQQSAGG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
- assigned_chemical_shifts_2

Data type	Count
13C chemical shifts	637
15N chemical shifts	190
1H chemical shifts	190

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TOG D1	1

Entities:

Entity 1, TOG D1 250 residues - Formula weight is not available

1	MET	GLY	ASP	ASP	SER	GLU	TRP	LEU	LYS	LEU
2	PRO	VAL	ASP	GLN	LYS	CYS	GLU	HIS	LYS	LEU
3	TRP	LYS	ALA	ARG	LEU	SER	GLY	TYR	GLU	GLU
4	ALA	LEU	LYS	ILE	PHE	GLN	LYS	ILE	LYS	ASP
5	GLU	LYS	SER	PRO	GLU	TRP	SER	LYS	PHE	LEU
6	GLY	LEU	ILE	LYS	LYS	PHE	VAL	THR	ASP	SER
7	ASN	ALA	VAL	VAL	GLN	LEU	LYS	GLY	LEU	GLU
8	ALA	ALA	LEU	VAL	TYR	VAL	GLU	ASN	ALA	HIS
9	VAL	ALA	GLY	LYS	THR	THR	GLY	GLU	VAL	VAL
10	SER	GLY	VAL	VAL	SER	LYS	VAL	PHE	ASN	GLN
11	PRO	LYS	ALA	LYS	ALA	LYS	GLU	LEU	GLY	ILE
12	GLU	ILE	CYS	LEU	MET	TYR	ILE	GLU	ILE	GLU
13	LYS	GLY	GLU	ALA	VAL	GLN	GLU	GLU	LEU	LEU
14	LYS	GLY	LEU	ASP	ASN	LYS	ASN	PRO	LYS	ILE
15	ILE	VAL	ALA	CYS	ILE	GLU	THR	LEU	ARG	LYS
16	ALA	LEU	SER	GLU	PHE	GLY	SER	LYS	ILE	ILE
17	LEU	LEU	LYS	PRO	ILE	ILE	LYS	VAL	LEU	PRO
18	LYS	LEU	PHE	GLU	SER	ARG	GLU	LYS	ALA	VAL
19	ARG	ASP	GLU	ALA	LYS	LEU	ILE	ALA	VAL	GLU
20	ILE	TYR	ARG	TRP	ILE	ARG	ASP	ALA	LEU	ARG
21	PRO	PRO	LEU	GLN	ASN	ILE	ASN	SER	VAL	GLN
22	LEU	LYS	GLU	LEU	GLU	GLU	GLU	TRP	VAL	LYS
23	LEU	PRO	THR	SER	ALA	PRO	ARG	PRO	THR	ARG
24	PHE	LEU	ARG	SER	GLN	GLN	GLU	LEU	GLU	ALA
25	LYS	LEU	GLU	GLN	GLN	GLN	SER	ALA	GLY	GLY

Samples:

sample_1: TOG D1, [U-100% 13C; U-100% 15N; U-80% 2H], 250 uM; DTT 1 mM; D2O, [U-99% 2H], 10%; MES 20 mM

sample_conditions_1: ionic strength: 0 M; pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
HNCACO	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - data analysis

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

Bruker Avance 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

1	MET	GLY	ASP	ASP	SER	GLU	TRP	LEU	LYS	LEU
2	PRO	VAL	ASP	GLN	LYS	CYS	GLU	HIS	LYS	LEU
3	TRP	LYS	ALA	ARG	LEU	SER	GLY	TYR	GLU	GLU
4	ALA	LEU	LYS	ILE	PHE	GLN	LYS	ILE	LYS	ASP
5	GLU	LYS	SER	PRO	GLU	TRP	SER	LYS	PHE	LEU
6	GLY	LEU	ILE	LYS	LYS	PHE	VAL	THR	ASP	SER
7	ASN	ALA	VAL	VAL	GLN	LEU	LYS	GLY	LEU	GLU
8	ALA	ALA	LEU	VAL	TYR	VAL	GLU	ASN	ALA	HIS
9	VAL	ALA	GLY	LYS	THR	THR	GLY	GLU	VAL	VAL
10	SER	GLY	VAL	VAL	SER	LYS	VAL	PHE	ASN	GLN
11	PRO	LYS	ALA	LYS	ALA	LYS	GLU	LEU	GLY	ILE
12	GLU	ILE	CYS	LEU	MET	TYR	ILE	GLU	ILE	GLU
13	LYS	GLY	GLU	ALA	VAL	GLN	GLU	GLU	LEU	LEU
14	LYS	GLY	LEU	ASP	ASN	LYS	ASN	PRO	LYS	ILE
15	ILE	VAL	ALA	CYS	ILE	GLU	THR	LEU	ARG	LYS
16	ALA	LEU	SER	GLU	PHE	GLY	SER	LYS	ILE	ILE
17	LEU	LEU	LYS	PRO	ILE	ILE	LYS	VAL	LEU	PRO
18	LYS	LEU	PHE	GLU	SER	ARG	GLU	LYS	ALA	VAL
19	ARG	ASP	GLU	ALA	LYS	LEU	ILE	ALA	VAL	GLU
20	ILE	TYR	ARG	TRP	ILE	ARG	ASP	ALA	LEU	ARG
21	PRO	PRO	LEU	GLN	ASN	ILE	ASN	SER	VAL	GLN
22	LEU	LYS	GLU	LEU	GLU	GLU	GLU	TRP	VAL	LYS
23	LEU	PRO	THR	SER	ALA	PRO	ARG	PRO	THR	ARG
24	PHE	LEU	ARG	SER	GLN	GLN	GLU	LEU	GLU	ALA
25	LYS	LEU	GLU	GLN	GLN	GLN	SER	ALA	GLY	GLY

1	MET	GLY	ASP	ASP	SER	GLU	TRP	LEU	LYS	LEU
2	PRO	VAL	ASP	GLN	LYS	CYS	GLU	HIS	LYS	LEU
3	TRP	LYS	ALA	ARG	LEU	SER	GLY	TYR	GLU	GLU
4	ALA	LEU	LYS	ILE	PHE	GLN	LYS	ILE	LYS	ASP
5	GLU	LYS	SER	PRO	GLU	TRP	SER	LYS	PHE	LEU
6	GLY	LEU	ILE	LYS	LYS	PHE	VAL	THR	ASP	SER
7	ASN	ALA	VAL	VAL	GLN	LEU	LYS	GLY	LEU	GLU
8	ALA	ALA	LEU	VAL	TYR	VAL	GLU	ASN	ALA	HIS
9	VAL	ALA	GLY	LYS	THR	THR	GLY	GLU	VAL	VAL
10	SER	GLY	VAL	VAL	SER	LYS	VAL	PHE	ASN	GLN
11	PRO	LYS	ALA	LYS	ALA	LYS	GLU	LEU	GLY	ILE
12	GLU	ILE	CYS	LEU	MET	TYR	ILE	GLU	ILE	GLU
13	LYS	GLY	GLU	ALA	VAL	GLN	GLU	GLU	LEU	LEU
14	LYS	GLY	LEU	ASP	ASN	LYS	ASN	PRO	LYS	ILE
15	ILE	VAL	ALA	CYS	ILE	GLU	THR	LEU	ARG	LYS
16	ALA	LEU	SER	GLU	PHE	GLY	SER	LYS	ILE	ILE
17	LEU	LEU	LYS	PRO	ILE	ILE	LYS	VAL	LEU	PRO
18	LYS	LEU	PHE	GLU	SER	ARG	GLU	LYS	ALA	VAL
19	ARG	ASP	GLU	ALA	LYS	LEU	ILE	ALA	VAL	GLU
20	ILE	TYR	ARG	TRP	ILE	ARG	ASP	ALA	LEU	ARG
21	PRO	PRO	LEU	GLN	ASN	ILE	ASN	SER	VAL	GLN
22	LEU	LYS	GLU	LEU	GLU	GLU	GLU	TRP	VAL	LYS
23	LEU	PRO	THR	SER	ALA	PRO	ARG	PRO	THR	ARG
24	PHE	LEU	ARG	SER	GLN	GLN	GLU	LEU	GLU	ALA
25	LYS	LEU	GLU	GLN	GLN	GLN	SER	ALA	GLY	GLY

1	MET	GLY	ASP	ASP	SER	GLU	TRP	LEU	LYS	LEU
2	PRO	VAL	ASP	GLN	LYS	CYS	GLU	HIS	LYS	LEU
3	TRP	LYS	ALA	ARG	LEU	SER	GLY	TYR	GLU	GLU
4	ALA	LEU	LYS	ILE	PHE	GLN	LYS	ILE	LYS	ASP
5	GLU	LYS	SER	PRO	GLU	TRP	SER	LYS	PHE	LEU
6	GLY	LEU	ILE	LYS	LYS	PHE	VAL	THR	ASP	SER
7	ASN	ALA	VAL	VAL	GLN	LEU	LYS	GLY	LEU	GLU
8	ALA	ALA	LEU	VAL	TYR	VAL	GLU	ASN	ALA	HIS
9	VAL	ALA	GLY	LYS	THR	THR	GLY	GLU	VAL	VAL
10	SER	GLY	VAL	VAL	SER	LYS	VAL	PHE	ASN	GLN
11	PRO	LYS	ALA	LYS	ALA	LYS	GLU	LEU	GLY	ILE
12	GLU	ILE	CYS	LEU	MET	TYR	ILE	GLU	ILE	GLU
13	LYS	GLY	GLU	ALA	VAL	GLN	GLU	GLU	LEU	LEU
14	LYS	GLY	LEU	ASP	ASN	LYS	ASN	PRO	LYS	ILE
15	ILE	VAL	ALA	CYS	ILE	GLU	THR	LEU	ARG	LYS
16	ALA	LEU	SER	GLU	PHE	GLY	SER	LYS	ILE	ILE
17	LEU	LEU	LYS	PRO	ILE	ILE	LYS	VAL	LEU	PRO
18	LYS	LEU	PHE	GLU	SER	ARG	GLU	LYS	ALA	VAL
19	ARG	ASP	GLU	ALA	LYS	LEU	ILE	ALA	VAL	GLU
20	ILE	TYR	ARG	TRP	ILE	ARG	ASP	ALA	LEU	ARG
21	PRO	PRO	LEU	GLN	ASN	ILE	ASN	SER	VAL	GLN
22	LEU	LYS	GLU	LEU	GLU	GLU	GLU	TRP	VAL	LYS
23	LEU	PRO	THR	SER	ALA	PRO	ARG	PRO	THR	ARG
24	PHE	LEU	ARG	SER	GLN	GLN	GLU	LEU	GLU	ALA
25	LYS	LEU	GLU	GLN	GLN	GLN	SER	ALA	GLY	GLY