BMRB Entry 50253

Title:
4E-BP2 chemical shift data for SSP calculation
Deposition date:
2020-04-30
Original release date:
2020-05-12
Authors:
Dawson, Jennifer; Bah, Alaji; Zhang, Zhenfu; Vernon, Robert; Lin, Hong; Chong, Andrew; Vanama, Manasvi; Sonenberg, Nahum; Gradinaru, Claudiu; Forman-Kay, Julie
Citation:

Citation: Dawson, Jennifer; Bah, Alaji; Zhang, Zhenfu; Vernon, Robert; Lin, Hong; Chong, Andrew; Vanama, Manasvi; Sonenberg, Nahum; Gradinaru, Claudiu; Forman-Kay, Julie. "Non-cooperative 4E-BP2 folding with exchange between eIF4E-binding and binding-incompatible states tunes cap-dependent translation inhibition"  Nat. Commun. 11, 3146-3146 (2020).
PubMed: 32561718

Assembly members:

Assembly members:
entity_1, polymer, 120 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET His_SUMO

Data typeCount
13C chemical shifts1206
15N chemical shifts395
1H chemical shifts845

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
14E-BP21

Entities:

Entity 1, 4E-BP2 120 residues - Formula weight is not available

1   METSERSERSERALAGLYSERGLYHISGLN
2   PROSERGLNSERARGALAILEPROTHRARG
3   THRVALALAILESERASPALAALAGLNLEU
4   PROHISASPTYRCYSTHRTHRPROGLYGLY
5   THRLEUPHESERTHRTHRPROGLYGLYTHR
6   ARGILEILETYRASPARGLYSPHELEULEU
7   ASPARGARGASNSERPROMETALAGLNTHR
8   PROPROCYSHISLEUPROASNILEPROGLY
9   VALTHRSERPROGLYTHRLEUILEGLUASP
10   SERLYSVALGLUVALASNASNLEUASNASN
11   LEUASNASNHISASPARGLYSHISALAVAL
12   GLYASPASPALAGLNPHEGLUMETASPILE

Samples:

sample_1: 4E-BP2, [U-100% 13C; U-100% 15N], 0.75 mM

sample_conditions_1: pH: 7.4; temperature: 293 K

sample_conditions_2: pH: 3.0; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_2
3D HNCOsample_1isotropicsample_conditions_2
3D CBCA(CO)NHsample_1isotropicsample_conditions_2
3D HNCACBsample_1isotropicsample_conditions_2
3D HBHA(CO)NHsample_1isotropicsample_conditions_2
3D 1H-15N NOESYsample_1isotropicsample_conditions_2

Software:

SPARKY - chemical shift assignment

NMR spectrometers:

  • Varian Uniform NMR System 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks