BMRB Entry 50168

Title:
J-domain region of DnaJA2
Deposition date:
2020-01-26
Original release date:
2020-11-11
Authors:
Rosenzweig, Rina; Abayev-Avraham, Meital
Citation:

Citation: Rosenzweig, Rina; Abayev-Avraham, Maital; Faust, Ofrah; Maurer, Michael; Wentink, Anne; Bukau, Bernd; Nillegoda, Nadinath; London, Nir. "Hsp40s employ class-specific regulation to drive Hsp70 functional diversity"  Nature 587, 489-494 (2020).
PubMed: 33177718

Assembly members:

Assembly members:
J-domain of DnaJA2, polymer, 77 residues, 8777 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET29b

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts204
15N chemical shifts69
1H chemical shifts69

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1J-domain region of DnaJA21

Entities:

Entity 1, J-domain region of DnaJA2 77 residues - 8777 Da.

1   METALAASNVALALAASPTHRLYSLEUTYR
2   ASPILELEUGLYVALPROPROGLYALASER
3   GLUASNGLULEULYSLYSALATYRARGLYS
4   LEUALALYSGLUTYRHISPROASPLYSASN
5   PROASNALAGLYASPLYSPHELYSGLUILE
6   SERPHEALATYRGLUVALLEUSERASNPRO
7   GLULYSARGGLULEUTYRASPARGTYRGLY
8   GLUGLNGLYLEUARGGLUGLY

Samples:

sample_1: HEPES 50 mM; KCl 50 mM; sodium azide 0.03%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 50 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1

Software:

TOPSPIN v4.0.6, Bruker Biospin - collection, processing

CcpNMR v2.4.2, CCPN - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks