BMRB Entry 50154

Title:
C-terminal domain of TDP-43 at acidic pH
Deposition date:
2020-01-09
Original release date:
2021-03-10
Authors:
Mompean, Miguel
Citation:

Citation: Pantoja-Uceda, David; Stuani, Cristiana; Laurents, Douglas; McDermott, Ann; Buratti, Emanuele; Mompean, Miguel. "NMR assignments for the C-terminal domain of human TDP-43"  Biomol. NMR Assign. 15, 177-181 (2021).
PubMed: 33417141

Assembly members:

Assembly members:
TDP-43 267-414, polymer, 169 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pJ411

Data sets:
Data typeCount
13C chemical shifts467
15N chemical shifts163
1H chemical shifts552

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C-terminal domain of TDP-431

Entities:

Entity 1, C-terminal domain of TDP-43 169 residues - Formula weight is not available

1   METGLYSERASPLYSILEHISHISHISHIS
2   HISHISGLUASNLEUTYRPHEGLNGLYHIS
3   METASNARGGLNLEUGLUARGSERGLYARG
4   PHEGLYGLYASNPROGLYGLYPHEGLYASN
5   GLNGLYGLYPHEGLYASNSERARGGLYGLY
6   GLYALAGLYLEUGLYASNASNGLNGLYSER
7   ASNMETGLYGLYGLYMETASNPHEGLYALA
8   PHESERILEASNPROALAMETMETALAALA
9   ALAGLNALAALALEUGLNSERSERTRPGLY
10   METMETGLYMETLEUALASERGLNGLNASN
11   GLNSERGLYPROSERGLYASNASNGLNASN
12   GLNGLYASNMETGLNARGGLUPROASNGLN
13   ALAPHEGLYSERGLYASNASNSERTYRSER
14   GLYSERASNSERGLYALAALAILEGLYTRP
15   GLYSERALASERASNALAGLYSERGLYSER
16   GLYPHEASNGLYGLYPHEGLYSERSERMET
17   ASPSERLYSSERSERGLYTRPGLYMET

Samples:

sample_1: entity_1, [U-100% 13C; U-100% 15N], 60-120 uM

sample_conditions_1: pH: 4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CC(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(NCOCA)NHsample_1isotropicsample_conditions_1
3D (H)N(COCA)NHsample_1isotropicsample_conditions_1

Software:

CcpNMR, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks