BMRB Entry 5012

Title:
Gelatin-binding Region of Human Matrix Metalloproteinase-2: Solution Structure, Dynamics, and Function of the COL-23 Two-domain Construct
Deposition date:
2001-05-18
Original release date:
2001-09-13
Authors:
Briknarova, Klara; Gehrmann, Marion; Banyai, Laszlo; Tordai, Hedwig; Patthy, Laszlo; Llinas, Miguel
Citation:

Citation: Briknarova, Klara; Gehrmann, Marion; Banyai, Laszlo; Tordai, Hedwig; Patthy, Laszlo; Llinas, Miguel. "Gelatin-binding Region of Human Matrix Metalloproteinase-2: Solution Structure, Dynamics, and Function of the COL-23 Two-domain Construct"  J. Biol. Chem. 276, 27613-27621 (2001).
PubMed: 11320090

Assembly members:

Assembly members:
Matrix Metalloproteinase 2, polymer, 72 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Matrix Metalloproteinase 2: LAAHPPFASWMSTVGGNSGG APCVFPFTFLGNKYESCTSA GRSDGKMWCATTANYDDDRK WGFCPDQGWISS

Data sets:
  • assigned_chemical_shifts
Data typeCount
15N chemical shifts75
1H chemical shifts424

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1col-3 domain, MMP-21

Entities:

Entity 1, col-3 domain, MMP-2 72 residues - Formula weight is not available

1   LEUALAALAHISPROPROPHEALASERTRP
2   METSERTHRVALGLYGLYASNSERGLYGLY
3   ALAPROCYSVALPHEPROPHETHRPHELEU
4   GLYASNLYSTYRGLUSERCYSTHRSERALA
5   GLYARGSERASPGLYLYSMETTRPCYSALA
6   THRTHRALAASNTYRASPASPASPARGLYS
7   TRPGLYPHECYSPROASPGLNGLYTRPILE
8   SERSER

Samples:

sample_1: Matrix Metalloproteinase 2 0.5 mM

sample_3: Matrix Metalloproteinase 2, [U-15N], 1 mM

Ex-cond_1: pH: 5.4; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D COSYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
3D 15N NOESYnot availablenot availablenot available
3D 15N TOCSYnot availablenot availablenot available
HNHBnot availablenot availablenot available
HSQCnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks