BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 50117

Title: UN2A

Deposition date: 2019-12-06 Original release date: 2021-03-18

Authors: Zhou, Tiankun

Citation: Zhou, Tiankun; Fleming, Jennifer; Bogomolovas, Julius; Stronczek, Chiara; Grundei, David; Ghassemian, Majid; Biju, Andrea; Borgeson, Emma; Lange, Stephan; Bullard, Belinda; Kovermann, Michael; Mayans, Olga. "Molecular characterization of titin N2A and its interaction with CARP"  .

Assembly members:
entity_1, polymer, 111 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-Trx-1a

Entity Sequences (FASTA):
entity_1: MDERKKQEKIEGDLRAMLKK TPILKKGAGEEEEIDIMELL KNVDPKEYEKYARMYGITDF RGLLQAFELLKQSQEEETHR LEIEEIERSERDEKEFEELV SFIQQRLSQTE

Data sets:
Data typeCount
13C chemical shifts253
15N chemical shifts75
1H chemical shifts75

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UN2A1

Entities:

Entity 1, UN2A 111 residues - Formula weight is not available

1   METASPGLUARGLYSLYSGLNGLULYSILE
2   GLUGLYASPLEUARGALAMETLEULYSLYS
3   THRPROILELEULYSLYSGLYALAGLYGLU
4   GLUGLUGLUILEASPILEMETGLULEULEU
5   LYSASNVALASPPROLYSGLUTYRGLULYS
6   TYRALAARGMETTYRGLYILETHRASPPHE
7   ARGGLYLEULEUGLNALAPHEGLULEULEU
8   LYSGLNSERGLNGLUGLUGLUTHRHISARG
9   LEUGLUILEGLUGLUILEGLUARGSERGLU
10   ARGASPGLULYSGLUPHEGLUGLULEUVAL
11   SERPHEILEGLNGLNARGLEUSERGLNTHR
12   GLU

Samples:

sample_1: UN2A, [U-13C; U-15N; U-2H], 1.2 mM; sodium phosphate 30 mM; sodium chloride 100 mM

sample_conditions_1: pH: 7.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRViewJ, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts