BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 50110

Title: Sequential solid-state NMR assignment of Snu13p in Snu13p:Rsa1p:Hit1p complex   PubMed: 32030621

Deposition date: 2019-12-03 Original release date: 2019-12-23

Authors: Gardiennet, Carole; Quinternet, Marc; Manival, Xavier; Chagot, Marie-Eve

Citation: Chagot, Marie-Eve; Quinternet, Marc; Jacquemin, Clemence; Manival, Xavier; Gardiennet, Carole. "Box C/D snoRNPs: Solid-State NMR Fingerprint of an Early-Stage 50 kDa Assembly Intermediate"  Biomol. NMR Assignments 14, 131-140 (2020).

Assembly members:
entity_1, polymer, 129 residues, 13568.90 Da.
entity_2, polymer, 118 residues, 14072.15 Da.
entity_3, polymer, 95 residues, Formula weight is not available

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pnEA

Entity Sequences (FASTA):
entity_1: GPHMSAPNPKAFPLADAALT QQILDVVQQAANLRQLKKGA NEATKTLNRGISEFIIMAAD CEPIEILLHLPLLCEDKNVP YVFVPSRVALGRACGVSRPV IAASITTNDASAIKTQIYAV KDKIETLLI
entity_2: GPHMDEDVKKWREERKKMWL LKISNNKQKHMQEMGIKEDE LKSQPSIFKESRKEKQFIQS IQNQVQRGNPKIDLNLKLIQ REFANENSQLLDFIRELGDV GLLEYELSQQEKDVLFGS
entity_3: MNKTLKTKAFDDIYQNSAEL QELLKYNTVKFHLAKVYRIL SSTVNDGSSGKMNSDLQKEL AVNYLNTLRYGGIHYNEAIE EFCQILLDKLNAVKK

Data sets:
Data typeCount
13C chemical shifts410
15N chemical shifts106

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Snu13p1
2Rsa1p(238-352)2
3Hit1p(70-164)3

Entities:

Entity 1, Snu13p 129 residues - 13568.90 Da.

1   GLYPROHISMETSERALAPROASNPROLYS
2   ALAPHEPROLEUALAASPALAALALEUTHR
3   GLNGLNILELEUASPVALVALGLNGLNALA
4   ALAASNLEUARGGLNLEULYSLYSGLYALA
5   ASNGLUALATHRLYSTHRLEUASNARGGLY
6   ILESERGLUPHEILEILEMETALAALAASP
7   CYSGLUPROILEGLUILELEULEUHISLEU
8   PROLEULEUCYSGLUASPLYSASNVALPRO
9   TYRVALPHEVALPROSERARGVALALALEU
10   GLYARGALACYSGLYVALSERARGPROVAL
11   ILEALAALASERILETHRTHRASNASPALA
12   SERALAILELYSTHRGLNILETYRALAVAL
13   LYSASPLYSILEGLUTHRLEULEUILE

Entity 2, Rsa1p(238-352) 118 residues - 14072.15 Da.

1   GLYPROHISMETASPGLUASPVALLYSLYS
2   TRPARGGLUGLUARGLYSLYSMETTRPLEU
3   LEULYSILESERASNASNLYSGLNLYSHIS
4   METGLNGLUMETGLYILELYSGLUASPGLU
5   LEULYSSERGLNPROSERILEPHELYSGLU
6   SERARGLYSGLULYSGLNPHEILEGLNSER
7   ILEGLNASNGLNVALGLNARGGLYASNPRO
8   LYSILEASPLEUASNLEULYSLEUILEGLN
9   ARGGLUPHEALAASNGLUASNSERGLNLEU
10   LEUASPPHEILEARGGLULEUGLYASPVAL
11   GLYLEULEUGLUTYRGLULEUSERGLNGLN
12   GLULYSASPVALLEUPHEGLYSER

Entity 3, Hit1p(70-164) 95 residues - Formula weight is not available

native

1   METASNLYSTHRLEULYSTHRLYSALAPHE
2   ASPASPILETYRGLNASNSERALAGLULEU
3   GLNGLULEULEULYSTYRASNTHRVALLYS
4   PHEHISLEUALALYSVALTYRARGILELEU
5   SERSERTHRVALASNASPGLYSERSERGLY
6   LYSMETASNSERASPLEUGLNLYSGLULEU
7   ALAVALASNTYRLEUASNTHRLEUARGTYR
8   GLYGLYILEHISTYRASNGLUALAILEGLU
9   GLUPHECYSGLNILELEULEUASPLYSLEU
10   ASNALAVALLYSLYS

Samples:

sample_1: Snu13p, [U-100% 13C; U-100% 15N], 200 mg/mL; Rsa1p(238-352) 200 mg/mL; Hit1p(70-164) 200 mg/mL; HEPES 10 mM; sodium chloride 150 mM; TCEP 0.5 mM; sodium azide 0.05 % w/v

sample_conditions_1: ionic strength: 150 mM; pH: 7.5; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D DARRsample_1isotropicsample_conditions_1
2D DARRsample_1isotropicsample_conditions_1
2D DREAMsample_1isotropicsample_conditions_1
2D NCAsample_1isotropicsample_conditions_1
2D NCOsample_1isotropicsample_conditions_1
3D NCACXsample_1isotropicsample_conditions_1
3D NCACXsample_1isotropicsample_conditions_1
3D NCACBsample_1isotropicsample_conditions_1
3D CANCOsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.5, Bruker Biospin - processing

CcpNMR v2.4, CCPN - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 950 MHz

Related Database Links:

SP P39990
UNP Q08932-1 P46973-1