BMRB Entry 50094

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50094

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Title:
The N-terminal PARP-like domain of TASOR
Deposition date:
2019-11-28
Original release date:
2021-08-13
Authors:
Douse, Christopher; Timms, Richard; Tchasovnikarova, Iva; Wagstaff, Jane; Freund, Stefan; Lehner, Paul; Modis, Yorgo
Citation:

Citation: Douse, Christopher; Tchasovnikarova, Iva; Timms, Richard; Protasio, Anna; Seczynska, Marta; Prigozhin, Daniil; Albecka, Anna; Wagstaff, Jane; Williamson, James; Freund, Stefan; Lehner, Paul; Modis, Yorgo. "TASOR is a pseudo-PARP that directs HUSH complex assembly and epigenetic transposon control"  Nat. Commun. 11, 4940-4940 (2020).
PubMed: 33009411

Assembly members:

Assembly members:
PARP-like domain of TASOR, polymer, 231 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PARP-like domain of TASOR: GSHMSREKKALFQPLTPGSR EFEDVVNIMHSSYLEPTSVT NFNYRRACLVHNELLEKEFT EKRRELKFDGRLDKELSESY AFLMVDRYQVQTICEKGLHV GQSKITILGSPSMGVYLSRY ADLLQANPLDTGAMGDVVIF KIMKGKIKSIYDPMGVKSLE SMLNKSALDPTPKHECHVSK NANRITSLLAYRAYELTQYY FYEYGFDELRRRPRHVCPYA VVSFTYKDDIQ

Data sets:
Data typeCount
13C chemical shifts522
15N chemical shifts169
1H chemical shifts169

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PARP-like domain of TASOR1

Entities:

Entity 1, PARP-like domain of TASOR 231 residues - Formula weight is not available

1   GLYSERHISMETSERARGGLULYSLYSALA
2   LEUPHEGLNPROLEUTHRPROGLYSERARG
3   GLUPHEGLUASPVALVALASNILEMETHIS
4   SERSERTYRLEUGLUPROTHRSERVALTHR
5   ASNPHEASNTYRARGARGALACYSLEUVAL
6   HISASNGLULEULEUGLULYSGLUPHETHR
7   GLULYSARGARGGLULEULYSPHEASPGLY
8   ARGLEUASPLYSGLULEUSERGLUSERTYR
9   ALAPHELEUMETVALASPARGTYRGLNVAL
10   GLNTHRILECYSGLULYSGLYLEUHISVAL
11   GLYGLNSERLYSILETHRILELEUGLYSER
12   PROSERMETGLYVALTYRLEUSERARGTYR
13   ALAASPLEULEUGLNALAASNPROLEUASP
14   THRGLYALAMETGLYASPVALVALILEPHE
15   LYSILEMETLYSGLYLYSILELYSSERILE
16   TYRASPPROMETGLYVALLYSSERLEUGLU
17   SERMETLEUASNLYSSERALALEUASPPRO
18   THRPROLYSHISGLUCYSHISVALSERLYS
19   ASNALAASNARGILETHRSERLEULEUALA
20   TYRARGALATYRGLULEUTHRGLNTYRTYR
21   PHETYRGLUTYRGLYPHEASPGLULEUARG
22   ARGARGPROARGHISVALCYSPROTYRALA
23   VALVALSERPHETHRTYRLYSASPASPILE
24   GLN

Samples:

sample_1: NaCl 150 mM; NaN3 0.05%; PARP-like domain of TASOR, [U-95% 15N], 100 uM; sodium phosphate 25 mM; TCEP 1 mM; D20, [U-99% 2H], 5%

sample_2: NaCl 150 mM; NaN3 0.05%; PARP-like domain of TASOR, [U-95% 13C; U-95% 15N], 100 uM; sodium phosphate 25 mM; TCEP 1 mM; D2O, [U-99% 2H], 5%

sample_3: NaCl 150 mM; NaN3 0.05%; PARP-like domain of TASOR, [U-13C; U-15N; U-2H], 100 uM; sodium phosphate 25 mM; TCEP 1 mM; D2O, [U-99% 2H], 5%

sample_conditions_1: ionic strength: 175 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
BEST-TROSYsample_1isotropicsample_conditions_1
trHNCOsample_3isotropicsample_conditions_1
trHNCACOsample_3isotropicsample_conditions_1
trHNCAsample_3isotropicsample_conditions_1
trHNCOCAsample_3isotropicsample_conditions_1
trHNCACBsample_3isotropicsample_conditions_1
trHNCOCACBsample_3isotropicsample_conditions_1

Software:

TOPSPIN v3.1, Bruker Biospin - collection, processing

MARS v2.1, Raghavendran Lakshmi Narayanan & Young-Sang Jung & Markus Zweckstetter - chemical shift assignment

SPARKY v3.115, Goddard - data analysis, peak picking

qMDD, Maxim Mayzel & Vladislav Orekhov - processing

NMR spectrometers:

  • Bruker Avance II+ 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks