BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 50086

Title: 1H, 15N, 13Ca, 13Cb assignments for yeast Gal4 AD residues 828-881

Deposition date: 2019-11-12 Original release date: 2021-03-11

Authors: Tuttle, Lisa

Citation: Tuttle, Lisa; Pacheco, Derek; Warfield, Linda; Hahn, Steven; Klevit, Rachel. "Mediator subunit Med15 dictates the conserved fuzzy binding mechanism of yeast transcription activators Gal4 and Gcn4"  Nat. Commun. ., .-..

Assembly members:
entity_1, polymer, 54 residues, Formula weight is not available

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: SHY823

Entity Sequences (FASTA):
entity_1: DDGNNSKPLSPGWTDQTAYN AFGITTGMFNTTTMDDVYNY LFDDEDTPPNPKKE

Data sets:
Data typeCount
13C chemical shifts142
15N chemical shifts46
1H chemical shifts44

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Gal4 AD1

Entities:

Entity 1, Gal4 AD 54 residues - Formula weight is not available

1   ASPASPGLYASNASNSERLYSPROLEUSER
2   PROGLYTRPTHRASPGLNTHRALATYRASN
3   ALAPHEGLYILETHRTHRGLYMETPHEASN
4   THRTHRTHRMETASPASPVALTYRASNTYR
5   LEUPHEASPASPGLUASPTHRPROPROASN
6   PROLYSLYSGLU

Samples:

sample_1: sodium phosphate 20 mM; sodium chloride 150 mM; EDTA 0.1 mM; PMSF 0.1 mM; DTT 5 mM; yeast Gal4 AD, [U-100% 13C; U-100% 15N], 560 uM

sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker Avance II 600 MHz MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts