BMRB Entry 50015

Title:
Assignment of Titin domain I83
Deposition date:
2019-09-12
Original release date:
2021-04-11
Authors:
Pfuhl, Mark
Citation:

Citation: Kelly, Colleen; Pace, Nicola; Gage, Matthew; Pfuhl, Mark. "Solution NMR structure of titin N2A region Ig domain I83 and its interaction with metal ions"  J. Mol. Biol. 433, 166977-166977 (2021).
PubMed: 33811919

Assembly members:

Assembly members:
Titin, polymer, 106 residues, 11900 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET151/D-TOPO

Data sets:
Data typeCount
13C chemical shifts452
15N chemical shifts107
1H chemical shifts711

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1I831

Entities:

Entity 1, I83 106 residues - 11900 Da.

1   GLYILEASPPROPHETHRALAGLUPROILE
2   GLNPHETHRLYSARGILEGLNASNILEVAL
3   VALSERGLUHISGLNSERALATHRPHEGLU
4   CYSGLUVALSERPHEASPASPALAILEVAL
5   THRTRPTYRLYSGLYPROTHRGLULEUTHR
6   GLUSERGLNLYSTYRASNPHEARGASNASP
7   GLYARGCYSHISTYRMETTHRILEHISASN
8   VALTHRPROASPASPGLUGLYVALTYRSER
9   VALILEALAARGLEUGLUPROARGGLYGLU
10   ALAARGSERTHRALAGLULEULYSGLYGLU
11   LEUARGSERGLYCYSX

Samples:

sample_1: I83, [U-98% 15N], 0.6 mM

sample_2: I83, [U-95% 13C; U-95% 15N], 0.8 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7.20; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D H(CCO)NHsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1

Software:

TOPSPIN v4.0.6, Bruker Biospin - collection, processing

CcpNMR v2.4, CCPN - data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 800 MHz

Related Database Links:

GB NM_001267550_2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks