BMRB Entry 4987

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4987

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Title:
Backbone 1H,13C and 15N chemical shift assignment for the maltotriose-bound state of 2H,13C,15N-labeled maltodextrin-binding protein
Deposition date:
2001-04-11
Original release date:
2001-09-12
Authors:
Evenas, Johan; Tugarinov, Vitali; Skrynnikov, Nikolai; Goto, Natalie; Muhandiram, Ranjith; Kay, Lewis
Citation:

Citation: Evenas, Johan; Tugarinov, Vitali; Skrynnikov, Nikolai; Goto, Natalie; Muhandiram, Ranjith; Kay, Lewis. "Ligand-induced Structural changes to Maltodextrin-binding Protein as Studied by Solution NMR Spectroscopy"  J. Mol. Biol. 309, 961-974 (2001).
PubMed: 11399072

Assembly members:

Assembly members:
maltodextrin-binding protein, polymer, 370 residues, 40694 Da.
MLR, non-polymer, 504.437 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
maltodextrin-binding protein: KTEEGKLVIWINGDKGYNGL AEVGKKFEKDTGIKVTVEHP DKLEEKFPQVAATGDGPDII FWAHDRFGGYAQSGLLAEIT PDKAFQDKLYPFTWDAVRYN GKLIAYPIAVEALSLIYNKD LLPNPPKTWEEIPALDKELK AKGKSALMFNLQEPYFTWPL IAADGGYAFKYENGKYDIKD VGVDNAGAKAGLTFLVDLIK NKHMNADTDYSIAEAAFNKG ETAMTINGPWAWSNIDTSKV NYGVTVLPTFKGQPSKPFVG VLSAGINAASPNKELAKEFL ENYLLTDEGLEAVNKDKPLG AVALKSYEEELAKDPRIAAT MENAQKGEIMPNIPQMSAFW YAVRTAVINAASGRQTVDEA LKDAQTRITK

Data sets:
Data typeCount
13C chemical shifts1043
15N chemical shifts341
1H chemical shifts341

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1maltodextrin-binding protein1
2maltotriose2

Entities:

Entity 1, maltodextrin-binding protein 370 residues - 40694 Da.

1   LYSTHRGLUGLUGLYLYSLEUVALILETRP
2   ILEASNGLYASPLYSGLYTYRASNGLYLEU
3   ALAGLUVALGLYLYSLYSPHEGLULYSASP
4   THRGLYILELYSVALTHRVALGLUHISPRO
5   ASPLYSLEUGLUGLULYSPHEPROGLNVAL
6   ALAALATHRGLYASPGLYPROASPILEILE
7   PHETRPALAHISASPARGPHEGLYGLYTYR
8   ALAGLNSERGLYLEULEUALAGLUILETHR
9   PROASPLYSALAPHEGLNASPLYSLEUTYR
10   PROPHETHRTRPASPALAVALARGTYRASN
11   GLYLYSLEUILEALATYRPROILEALAVAL
12   GLUALALEUSERLEUILETYRASNLYSASP
13   LEULEUPROASNPROPROLYSTHRTRPGLU
14   GLUILEPROALALEUASPLYSGLULEULYS
15   ALALYSGLYLYSSERALALEUMETPHEASN
16   LEUGLNGLUPROTYRPHETHRTRPPROLEU
17   ILEALAALAASPGLYGLYTYRALAPHELYS
18   TYRGLUASNGLYLYSTYRASPILELYSASP
19   VALGLYVALASPASNALAGLYALALYSALA
20   GLYLEUTHRPHELEUVALASPLEUILELYS
21   ASNLYSHISMETASNALAASPTHRASPTYR
22   SERILEALAGLUALAALAPHEASNLYSGLY
23   GLUTHRALAMETTHRILEASNGLYPROTRP
24   ALATRPSERASNILEASPTHRSERLYSVAL
25   ASNTYRGLYVALTHRVALLEUPROTHRPHE
26   LYSGLYGLNPROSERLYSPROPHEVALGLY
27   VALLEUSERALAGLYILEASNALAALASER
28   PROASNLYSGLULEUALALYSGLUPHELEU
29   GLUASNTYRLEULEUTHRASPGLUGLYLEU
30   GLUALAVALASNLYSASPLYSPROLEUGLY
31   ALAVALALALEULYSSERTYRGLUGLUGLU
32   LEUALALYSASPPROARGILEALAALATHR
33   METGLUASNALAGLNLYSGLYGLUILEMET
34   PROASNILEPROGLNMETSERALAPHETRP
35   TYRALAVALARGTHRALAVALILEASNALA
36   ALASERGLYARGGLNTHRVALASPGLUALA
37   LEULYSASPALAGLNTHRARGILETHRLYS

Entity 2, maltotriose - C18 H32 O16 - 504.437 Da.

1   MLR

Samples:

sample_1: maltodextrin-binding protein, [U-2H; U-15N; U-13C], 1.3 mM; maltotriose 1.3 mM

mltrmbp_std: ionic strength: 0.1 M; pH: 7.2; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
TROSY-versions were used of all experiments listed.sample_1not availablemltrmbp_std
All non-exchangeable hydrogens are deuterons so allsample_1not availablemltrmbp_std
triple-resonance experiments are 2H-decoupled.sample_1not availablemltrmbp_std

Software:

NMRView v4.1 - semi-automatic assignment using CACB module

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian Inova 500 MHz

Related Database Links:

BMRB 25237 4354 4986 6807 7114
PDB
DBJ BAB38440 BAE78036 BAG79849 BAI28296 BAI33473
EMBL CAP78494 CAQ34383 CAR01012 CAR05669 CAR10711
GB AAB59056 AAB86559 AAB87675 AAC43128 AAC77004
REF NP_290668 NP_313044 NP_418458 NP_709885 NP_756856
SP P0AEX9 P0AEY0
AlphaFold P0AEX9 P0AEY0

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks