BMRB Entry 4976

Title:
Antifungal and antibacterial mutant of heliomicin
Deposition date:
2001-03-20
Original release date:
2002-04-04
Authors:
Landon, C.; Vovelle, F.
Citation:

Citation: Lamberty, M.; Caille, A.; Landon, C.; Tassin-Moindrot, S.; Hetru, C.; Bulet, P.; Vovelle, F.. "Solution Structures of the Antifungal Heliomicin and a Selected Variant with both Antibacterial and Antifungal Activities"  Biochemistry 40, 11995-12003 (2001).

Assembly members:

Assembly members:
heliomicin-LL, polymer, 44 residues, 4727.4 Da.

Natural source:

Natural source:   Common Name: tobacco budworm   Taxonomy ID: 7102   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Heliothis virescens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
heliomicin-LL: DKLIGSCVWGAVNYTSDCNG ECLLRGYKGGHCGSFANVNC WCET

Data sets:
Data typeCount
1H chemical shifts273
coupling constants32

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1helio-LL1

Entities:

Entity 1, helio-LL 44 residues - 4727.4 Da.

1   ASPLYSLEUILEGLYSERCYSVALTRPGLY
2   ALAVALASNTYRTHRSERASPCYSASNGLY
3   GLUCYSLEULEUARGGLYTYRLYSGLYGLY
4   HISCYSGLYSERPHEALAASNVALASNCYS
5   TRPCYSGLUTHR

Samples:

sample_1: heliomicin-LL 4 mM; sodium acetate 40 mM

sample_cond_1: ionic strength: 40 mM; pH: 4.3; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H DQF-COSYnot availablenot availablenot available
TQF-COSYnot availablenot availablenot available
TOCSYnot availablenot availablenot available
NOESYnot availablenot availablenot available

Software:

UXNMR - data collection

XEASY - data analysis

DYANA - refinement

X-PLOR - refinement

NMR spectrometers:

  • Bruker AMX 500 MHz

Related Database Links:

PDB