BMRB Entry 4953

Title:
Assignment of 1H, 13C and 15N resonances of the PPIase domain of the trigger factor from Mycoplasma genitalium
Deposition date:
2001-02-02
Original release date:
2001-06-29
Authors:
Parac, Tatjana; Vogtherr, Martin; Marcus, Marcus; Pahl, Andreas; Rueterjans, Heinz; Griesinger, Christian; Fiebig, Klaus
Citation:

Citation: Parac, Tatjana; Vogtherr, Martin; Maurer, Marcus; Pahl, Andreas; Rueterjans, Heinz; Griesinger, Christian; Fiebig, Klaus. "Letter to the Editor: Assignment of 1H, 13C and 15N resonances of the PPIase domain of the trigger factor from Mycoplasma genitalium"  J. Biomol. NMR 20, 193-194 (2001).

Assembly members:

Assembly members:
Peptidyl-prolyl-cis/trans isomerase, polymer, 113 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mycoplasma genitalium   Taxonomy ID: 2097   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Mycoplasma genitalium

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts416
15N chemical shifts105
1H chemical shifts640
coupling constants76

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1trigger factor1

Entities:

Entity 1, trigger factor 113 residues - Formula weight is not available

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYSERGLULYSLEUALALYSTHRLYSSER
3   THRMETVALASPVALSERASPLYSLYSLEU
4   ALAASNGLYASPILEALAILEILEASPPHE
5   THRGLYILEVALASPASNLYSLYSLEUALA
6   SERALASERALAGLNASNTYRGLULEUTHR
7   ILEGLYSERASNSERPHEILELYSGLYPHE
8   GLUTHRGLYLEUILEALAMETLYSVALASN
9   GLNLYSLYSTHRLEUALALEUTHRPHEPRO
10   SERASPTYRHISVALLYSGLULEUGLNSER
11   LYSPROVALTHRPHEGLUVALVALLEULYS
12   ALAILELYS

Samples:

sample_1: Peptidyl-prolyl-cis/trans isomerase, [U-100% 15N], 2.0 mM; sodium phosphate 50 mM

sample_2: Peptidyl-prolyl-cis/trans isomerase, [U-100% 13C; U-100% 15N], 2.0 mM; sodium phosphate 50 mM

sample_3: Peptidyl-prolyl-cis/trans isomerase 2.0 mM; sodium phosphate 50 mM

Ex-cond_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
NOESYnot availablenot availableEx-cond_1
TOCSYnot availablenot availableEx-cond_1
15N-edited NOESYnot availablenot availableEx-cond_1
15N-edited TOCSYnot availablenot availableEx-cond_1
13C-edited NOESYnot availablenot availableEx-cond_1
HNCOnot availablenot availableEx-cond_1
HNCAnot availablenot availableEx-cond_1
HNCACBnot availablenot availableEx-cond_1
CBCA(CO)NHnot availablenot availableEx-cond_1
HCCH-TOCSYnot availablenot availableEx-cond_1
H(CC)(CO)NH-TOCSYnot availablenot availableEx-cond_1
(H)CC(CO)NH-TOCSYnot availablenot availableEx-cond_1
HNHAnot availablenot availableEx-cond_1

Software:

xwinnmr v2.6 - acquisition, processing

XEASY v1.3.14 - assignment

NMR spectrometers:

  • Bruker Avance DRX 800 MHz
  • Bruker Avance DRX 600 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks