BMRB Entry 4933

Title:
1H and 15N Chemical Shift Assignments for a Cys58Ser mutant of TFF1
Deposition date:
2000-12-22
Original release date:
2001-02-15
Authors:
Williams, Mark; Polshakov, Vladimir; Feeney, James
Citation:

Citation: Polshakov, Vladimir; Thomas, Frenkiel; Westley, Bruce; Chadwick, Mark; May, Felicity; Carr, Mark; Feeney, James. "NMR-based structural Studies of the pNR-2/pS2 single domain trefoil peptide"  Eur. J. Biochem. 233, 847-855 (1995).

Assembly members:

Assembly members:
Trefoil Factor Family 1, polymer, 60 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: Pezz18 (Pharmacia)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Trefoil Factor Family 1: EAQTETCTVAPRERQNCGFP GVTPSQCANKGCCFDDTVRG VPWCFYPNTIDVPPEEESEF

Data sets:
Data typeCount
1H chemical shifts386
15N chemical shifts59

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TFF1 mutant1

Entities:

Entity 1, TFF1 mutant 60 residues - Formula weight is not available

1   GLUALAGLNTHRGLUTHRCYSTHRVALALA
2   PROARGGLUARGGLNASNCYSGLYPHEPRO
3   GLYVALTHRPROSERGLNCYSALAASNLYS
4   GLYCYSCYSPHEASPASPTHRVALARGGLY
5   VALPROTRPCYSPHETYRPROASNTHRILE
6   ASPVALPROPROGLUGLUGLUSERGLUPHE

Samples:

sample_1: Trefoil Factor Family 1 1.8 mM; sodium phosphate 5 mM

sample_2: Trefoil Factor Family 1, [U-95% 15N], 1.3 mM; sodium phosphate 5 mM

cond_1: pH: 5.8; temperature: 298 K; ionic strength: 0.01 M

cond_2: pH: 5.8; temperature: 283 K; ionic strength: 0.01 M

Experiments:

NameSampleSample stateSample conditions
DQF-COSYnot availablenot availablenot available
TOCSYnot availablenot availablenot available
NOESYnot availablenot availablenot available
ROESYnot availablenot availablenot available
13C-1H HSQCnot availablenot availablenot available
13C-1H HSQC-TOCSYnot availablenot availablenot available
15N-1H HSQCnot availablenot availablenot available
15N-1H HSQC-NOESYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian UNITY 600 MHz
  • Varian UNITY plus 500 MHz

Related Database Links:

BMRB 4930
PDB
DBJ BAA95532 BAB13729
EMBL CAA25155 CAA28695 CAA36254
GB AAA52402 AAH32811 AAX36234 AAX36702 AAX41076
PRF 1502207A 1716375A
REF NP_003216 XP_008975839 XP_009433935
SP P04155
AlphaFold P04155

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks