BMRB Entry 4918

Title:
Letter to the Editor: Assignments of 1H and 15N resonances of the Pseudomonas aeruginosa K122-4 pilin monomer.
Deposition date:
2000-12-12
Original release date:
2001-01-04
Authors:
Keizer, David; Kalsiak, Michal; Suh, Jeong-Yong; Crump, Matthew; Irvin, Randall; Sykes, Brian
Citation:

Citation: Keizer, David; Kalsiak, Michal; Suh, Jeong-Yong; Crump, Matthew; Irvin, Randall; Sykes, Brian. "Letter to the Editor: Assignments of 1H and 15N resonances of the Pseudomonas aeruginosa K122-4 pilin monomer"  J. Biomol. NMR 19, 385-386 (2001).

Assembly members:

Assembly members:
Pseudomonas Type IV pilin, polymer, 129 residues, 13105 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 287   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
15N chemical shifts124
1H chemical shifts632
coupling constants80

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1pilin monomer1

Entities:

Entity 1, pilin monomer 129 residues - 13105 Da.

1   ALALEUGLUGLYTHRGLUPHEALAARGALA
2   GLNLEUSERGLUALAMETTHRLEUALASER
3   GLYLEULYSTHRLYSVALSERASPILEPHE
4   SERGLNASPGLYSERCYSPROALAASNTHR
5   ALAALATHRALAGLYILEGLULYSASPTHR
6   ASPILEASNGLYLYSTYRVALALALYSVAL
7   THRTHRGLYGLYTHRALAALAALASERGLY
8   GLYCYSTHRILEVALALATHRMETLYSALA
9   SERASPVALALATHRPROLEUARGGLYLYS
10   THRLEUTHRLEUTHRLEUGLYASNALAASP
11   LYSGLYSERTYRTHRTRPALACYSTHRSER
12   ASNALAASPASNLYSTYRLEUPROLYSTHR
13   CYSGLNTHRALATHRTHRTHRTHRPRO

Samples:

sample_1: Pseudomonas Type IV pilin 2.2 mM

Ex-con_1: pH: 5.0; temperature: 303 K

Ex-cond_2: pH: 5.05; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H DQFCOSYnot availablenot availablenot available
2D 1H-1H TOCSYnot availablenot availablenot available
2D 1H-1H NOESYnot availablenot availablenot available
2D 1H-13C CT HSQCnot availablenot availablenot available
2D 1H-15N HSQCnot availablenot availablenot available
3D 1H-1H-15N NOESYnot availablenot availablenot available
3D 1H-1H-15N TOCSYnot availablenot availablenot available
3D HNHAnot availablenot availablenot available

Software:

NMRPIPE v1.8 - Processing of Spectra

NMRView v4.1.2 - Analysis of spectra

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian UNITY 600 MHz

Related Database Links:

SWISS-PROT P17838
GenBank AAC63062
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks