BMRB Entry 4860

Title:
1H, 13C, and 15N Chemical Shift Assignments for human lymphocyte specific kinase (Lck) unique and SH3 domain
Deposition date:
2000-10-17
Original release date:
2000-12-07
Authors:
Briese, Lars; Hoffmann, Silke; Friedrich, Ute; Biesinger, Brigitte; Willbold, Dieter
Citation:

Citation: Briese, Lars; Hoffmann, Silke; Friedrich, Ute; Biesinger, Brigitte; Willbold, Dieter. "Letter to the Editor: Sequence-specific 1H, 13C and 15N resonance assignments of lymphocyte specific kinase unique and SH3 domains"  J. Biomol. NMR 19, 193-194 (2001).

Assembly members:

Assembly members:
lymphocyte specific kinase, polymer, 126 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pLCKU3

Data sets:
Data typeCount
1H chemical shifts622
13C chemical shifts436
15N chemical shifts118

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Lck unique SH31

Entities:

Entity 1, Lck unique SH3 126 residues - Formula weight is not available

1   GLYPROLEUGLYSERMETGLYCYSGLYCYS
2   SERSERHISPROGLUASPASPTRPMETGLU
3   ASNILEASPVALCYSGLUASNCYSHISTYR
4   PROILEVALPROLEUASPGLYLYSGLYTHR
5   LEULEUILEARGASNGLYSERGLUVALARG
6   ASPPROLEUVALTHRTYRGLUGLYSERASN
7   PROPROALASERPROLEUGLNASPASNLEU
8   VALILEALALEUHISSERTYRGLUPROSER
9   HISASPGLYASPLEUGLYPHEGLULYSGLY
10   GLUGLNLEUARGILELEUGLUGLNSERGLY
11   GLUTRPTRPLYSALAGLNSERLEUTHRTHR
12   GLYGLNGLUGLYPHEILEPROPHEASNPHE
13   VALALALYSALAASNSER

Samples:

sample_1: lymphocyte specific kinase, [U-15N; U-13C], 1.6 mM; sodium acetate 20 mM; 2-mercaptoethanol 10 mM

Ex-cond_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N-HSQCnot availablenot availablenot available
13C-HSQCnot availablenot availablenot available
15N NOESY-HSQCnot availablenot availablenot available
13C NOESY-HSQCnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCACONHnot availablenot availablenot available
HCCONHnot availablenot availablenot available
CCONHnot availablenot availablenot available
HNCOnot availablenot availablenot available
HCCH-COSYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian Unity Inova 750 MHz

Related Database Links:

BMRB 5794
PDB
DBJ BAG64189 BAI45973
EMBL CAA28691 CAA31884 CAA32211 CAC44027
GB AAA18225 AAA59502 AAC50287 AAF34794 AAH13200
PRF 2201317A
REF NP_001036236 NP_005347 XP_002802373 XP_003276479 XP_003828198
SP P06239
TPE CAD55807
AlphaFold P06239

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks