BMRB Entry 4851

Title:
CDC4P from Schizosaccharomyces pombe
Deposition date:
2000-10-06
Original release date:
2000-10-23
Authors:
Slupsky, Carolyn; Desautels, Michel; Huebert, Terry; Zhao, Ruohong; Hemmingsen, Sean; Mcintosh, Lawrence
Citation:

Citation: Slupsky, Carolyn; Desautels, Michel; Huebert, Terry; Zhao, Ruohong; Hemmingsen, Sean; Mcintosh, Lawrence. "Structure of Cdc4p, a contractile ring protein essential for cytokinesis in Schizosaccharomyces pombe"  J. Biol. Chem. 276, 5943-5951 (2001).

Assembly members:

Assembly members:
EF-hand PROTEIN (CDC4P), polymer, 141 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: fission yeast   Taxonomy ID: 4896   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Schizosaccharomyces pombe

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PT7-7

Data sets:
Data typeCount
1H chemical shifts944
13C chemical shifts457
15N chemical shifts146

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CDC4p1

Entities:

Entity 1, CDC4p 141 residues - Formula weight is not available

1   METSERTHRASPASPSERPROTYRLYSGLN
2   ALAPHESERLEUPHEASPARGHISGLYTHR
3   GLYARGILEPROLYSTHRSERILEGLYASP
4   LEULEUARGALACYSGLYGLNASNPROTHR
5   LEUALAGLUILETHRGLUILEGLUSERTHR
6   LEUPROALAGLUVALASPMETGLUGLNPHE
7   LEUGLNVALLEUASNARGPROASNGLYPHE
8   ASPMETPROGLYASPPROGLUGLUPHEVAL
9   LYSGLYPHEGLNVALPHEASPLYSASPALA
10   THRGLYMETILEGLYVALGLYGLULEUARG
11   TYRVALLEUTHRSERLEUGLYGLULYSLEU
12   SERASNGLUGLUMETASPGLULEULEULYS
13   GLYVALPROVALLYSASPGLYMETVALASN
14   TYRHISASPPHEVALGLNMETILELEUALA
15   ASN

Samples:

sample_1: EF-hand PROTEIN (CDC4P), [U-15N], 4.5 – 5.5 mM; KCl 100 mM

sample_2: EF-hand PROTEIN (CDC4P), [U-15N; U-13C], 4.5 – 5.5 mM; KCl 100 mM

sample_cond_1: pH: 6.5; temperature: 303 K; ionic strength: 100 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D HNCACBnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D C(CO)NHnot availablenot availablenot available
3D HCCH-TOCSYnot availablenot availablenot available
3D 15N-TOCSY-HSQCnot availablenot availablenot available
3D 15N-NOESY-HSQCnot availablenot availablenot available
3D HNHBnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
2D COSYnot availablenot availablenot available
3D-HNHAnot availablenot availablenot available
3D 13C/15N-NOESY-HSQCnot availablenot availablenot available
4D 13C/13C-HMQC-NOESY-HMQCnot availablenot availablenot available

Software:

X-PLOR v3.8 - refinement, structure solution

NMR spectrometers:

  • Varian Unity 500 MHz

Related Database Links:

PDB
EMBL CAB55175
GB AAA67467
PRF 2119321A
REF NP_594947
SP Q09196
AlphaFold Q09196

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks