BMRB Entry 4840

Title:
1H, 13C and 15N resonance assignment and secondary structure of Mycobacterium tuberculosis adenylate kinase
Deposition date:
2000-09-26
Original release date:
2001-02-14
Authors:
Miron, Simona; Munier-Lehmann, Helene; Craescu, Constantin
Citation:

Citation: Miron, Simona; Munier-Lehmann, Helene; Craescu, Constantin. "Letter to the Editor: 1H, 13C and 15N resonance assignment and secondary structure of Mycobacterium tuberculosis adenylate kinase"  J. Biomol. NMR 19, 89-90 (2001).

Assembly members:

Assembly members:
Adenylate kinase from M. tuberculosis, polymer, 181 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 1773   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Mycobacterium tuberculosis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHL20

Data sets:
Data typeCount
1H chemical shifts848
13C chemical shifts333
15N chemical shifts178

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AK1

Entities:

Entity 1, AK 181 residues - Formula weight is not available

1   METARGVALLEULEULEUGLYPROPROGLY
2   ALAGLYLYSGLYTHRGLNALAVALLYSLEU
3   ALAGLULYSLEUGLYILEPROGLNILESER
4   THRGLYGLULEUPHEARGARGASNILEGLU
5   GLUGLYTHRLYSLEUGLYVALGLUALALYS
6   ARGTYRLEUASPALAGLYASPLEUVALPRO
7   SERASPLEUTHRASNGLULEUVALASPASP
8   ARGLEUASNASNPROASPALAALAASNGLY
9   PHEILELEUASPGLYTYRPROARGSERVAL
10   GLUGLNALALYSALALEUHISGLUMETLEU
11   GLUARGARGGLYTHRASPILEASPALAVAL
12   LEUGLUPHEARGVALSERGLUGLUVALLEU
13   LEUGLUARGLEULYSGLYARGGLYARGALA
14   ASPASPTHRASPASPVALILELEUASNARG
15   METLYSVALTYRARGASPGLUTHRALAPRO
16   LEULEUGLUTYRTYRARGASPGLNLEULYS
17   THRVALASPALAVALGLYTHRMETASPGLU
18   VALPHEALAARGALALEUARGALALEUGLY
19   LYS

Samples:

sample_one: Adenylate kinase from M. tuberculosis 1.0 mM; H2O 95%; D2O 5%

sample_two: Adenylate kinase from M. tuberculosis, [U-15N], 1.0 mM; H2O 95%; D2O 5%

sample_three: Adenylate kinase from M. tuberculosis, [U-15N; U-13C], 1.2 mM; H2O 95%; D2O 5%

sample_conditions_one: pH: 7.1; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Varian Unity 500 MHz
  • Varian Inova 750 MHz

Related Database Links:

PDB
DBJ BAH25046 BAL64618 BAQ04633 GAA44509
EMBL CAD93616 CAL70769 CCC25815 CCC43078 CCC63341
GB AAD09879 AAK44991 ABQ72469 ABR05090 ACT23783
REF NP_215247 NP_335177 NP_854412 WP_003403726 WP_003911271
SP A1KGL4 A5U0B9 C1AL67 P69439 P9WKF4
AlphaFold A1KGL4 A5U0B9 C1AL67 P69439 P9WKF4

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks