BMRB Entry 4832
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4832
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Citation: De Wolf, E.; Gill, R.; Geddes, S.; Pitts, J.; Wollmer, A.; Grotzinger, J.. "Solution Structure of a Mini IGF-1" Protein Sci. 5, 2193-2202 (1996).
Assembly members:
PROTEIN (GROWTH FACTOR IGF-1), polymer, 57 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
PROTEIN (GROWTH FACTOR IGF-1): GPETLCGAELVEALQFVCGE
RGFYFNKPGIVEECCFRSCE
LRRLEMYCAPLKPAKSA
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 375 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | IGF-1 | 1 |
Entities:
Entity 1, IGF-1 57 residues - Formula weight is not available
| 1 | GLY | PRO | GLU | THR | LEU | CYS | GLY | ALA | GLU | LEU | ||||
| 2 | VAL | GLU | ALA | LEU | GLN | PHE | VAL | CYS | GLY | GLU | ||||
| 3 | ARG | GLY | PHE | TYR | PHE | ASN | LYS | PRO | GLY | ILE | ||||
| 4 | VAL | GLU | GLU | CYS | CYS | PHE | ARG | SER | CYS | GLU | ||||
| 5 | LEU | ARG | ARG | LEU | GLU | MET | TYR | CYS | ALA | PRO | ||||
| 6 | LEU | LYS | PRO | ALA | LYS | SER | ALA |
Samples:
sample_1: PROTEIN (GROWTH FACTOR IGF-1) 2 mM
sample_cond_1: pH: 1.9; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H NOESY | not available | not available | not available |
| 1H TOCSY | not available | not available | not available |
Software:
Distance geometry vGEOMETRY - REFINEMENT
NMRView - Assignment
SNARF - Assignment
NMR spectrometers:
- Varian Unity 500 MHz