BMRB Entry 4743

Title:
1H Chemical Shift Assignments for fully reduced cytochrome c7 from Desulfuromonas acetoxidan
Deposition date:
2000-05-22
Original release date:
2000-07-13
Authors:
Assfalg, M.; Banci, L.; Bertini, I.; Bruschi, M.; Giudici-Orticoni, M.; Turano, P.
Citation:

Citation: Assfalg, M.; Banci, L.; Bertini, I.; Bruschi, M.; Giudici-Orticoni, M.; Turano, P.. "A proton-NMR investigation of the fully reduced cytochrome c7 from Desulfuromonas acetoxidans. Comparison between the reduced and the oxidized forms."  Eur. J. Biochem. 266, 634-643 (1999).

Assembly members:

Assembly members:
CYTOCHROME C7, polymer, 68 residues, 9500 Da.
HEC, non-polymer, 618.503 Da.

Natural source:

Natural source:   Common Name: Desulfuromonas acetoxidans   Taxonomy ID: 891   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Desulfuromonas acetoxidans

Experimental source:

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):

Data typeCount
1H chemical shifts449

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CYTOCHROME C71
2heme c, 12
3heme c, 22
4heme c, 32

Entities:

Entity 1, CYTOCHROME C7 68 residues - 9500 Da.

1   ALAASPVALVALTHRTYRGLUASNLYSLYS
2   GLYASNVALTHRPHEASPHISLYSALAHIS
3   ALAGLULYSLEUGLYCYSASPALACYSHIS
4   GLUGLYTHRPROALALYSILEALAILEASP
5   LYSLYSSERALAHISLYSASPALACYSLYS
6   THRCYSHISLYSSERASNASNGLYPROTHR
7   LYSCYSGLYGLYCYSHISILELYS

Entity 2, heme c, 1 - C34 H34 Fe N4 O4 - 618.503 Da.

1   HEC

Samples:

sample_1: CYTOCHROME C72 – 3 mM; sodium dithionite mM

sample_2: CYTOCHROME C72 – 3 mM

sample_cond_1: pH: 6.5; temperature: 292 K

sample_cond_2: pH: 6.5; temperature: 292 K

sample_cond_3: pH: 8.0; temperature: 292 K

Experiments:

NameSampleSample stateSample conditions
TOCSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available

Software:

AMBER v4.1 - refinement

DYANA v1.5 - structure solution

XEASY v3.1 - data analysis

XWINNMR - processing

NMR spectrometers:

  • Bruker AMX 500 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 4744
PDB
GB AAC46253
PRF 711089A
SP P00137
AlphaFold P00137 P00137