BMRB Entry 4704

Title:
Analysis of the dynamic properties of Bacillus circulans xylanase upon formation of a covalent glycosyl-enzyme intermediate
Deposition date:
2000-03-30
Original release date:
2000-05-08
Authors:
Connelly, Gregory; Withers, Stephen; McIntosh, Lawrence
Citation:

Citation: Connelly, Gregory; Withers, Stephen; McIntosh, Lawrence. "Analysis of the dynamic properties of Bacillus circulans xylanase upon formation of a covalent glycosyl-enzyme intermediate"  Protein Sci. 9, 512-524 (2000).

Assembly members:

Assembly members:
xylanase, polymer, 185 residues, 20396 Da.

Natural source:

Natural source:   Common Name: Bacillus circulans   Taxonomy ID: 1397   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Bacillus circulans

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Data sets:
Data typeCount
1H chemical shifts177
13C chemical shifts341
15N chemical shifts177

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BCX1

Entities:

Entity 1, BCX 185 residues - 20396 Da.

1   ALASERTHRASPTYRTRPGLNASNTRPTHR
2   ASPGLYGLYGLYILEVALASNALAVALASN
3   GLYSERGLYGLYASNTYRSERVALASNTRP
4   SERASNTHRGLYASNPHEVALVALGLYLYS
5   GLYTRPTHRTHRGLYSERPROPHEARGTHR
6   ILEASNTYRASNALAGLYVALTRPALAPRO
7   ASNGLYASNGLYTYRLEUTHRLEUTYRGLY
8   TRPTHRARGSERPROLEUILEGLUTYRTYR
9   VALVALASPSERTRPGLYTHRTYRARGPRO
10   THRGLYTHRTYRLYSGLYTHRVALLYSSER
11   ASPGLYGLYTHRTYRASPILETYRTHRTHR
12   THRARGTYRASNALAPROSERILEASPGLY
13   ASPARGTHRTHRPHETHRGLNTYRTRPSER
14   VALARGGLNTHRLYSARGPROTHRGLYSER
15   ASNALATHRILETHRPHESERASNHISVAL
16   ASNALATRPLYSSERHISGLYMETASNLEU
17   GLYSERASNTRPALATYRGLNVALMETALA
18   THRGLUGLYTYRGLNSERSERGLYSERSER
19   ASNVALTHRVALTRP

Samples:

apo-BCX: xylanase, [U-99% 13C; U-99% 15N], 0.9 mM

Conditions_1: pH: 5.7; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
HNCACBapo-BCXnot availableConditions_1
CBCACONHapo-BCXnot availableConditions_1

Software:

Felix v95 - data processing peak assignments

NMR spectrometers:

  • Varian Unity 500 MHz

Related Database Links:

BMRB 4705
PDB
DBJ BAH28803 BAM52542 BAM58117
EMBL CAA30553 CAA41783 CAA84276 CAB13776 CCU58510
GB AAA22897 AAB84458 AAM08359 AAM08360 AAW83159
PRF 1209158A
REF NP_389765 WP_003231377 WP_014477026 WP_014664226 WP_015251938
SP P09850 P18429
AlphaFold P18429 P09850

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks