BMRB Entry 4683

Title:
Multiple Modes of Peptide Recognition by the PTB Domain of the cell fate Determinant Numb
Deposition date:
2000-03-08
Original release date:
2000-06-19
Authors:
Zwahlen, Catherine; Li, Shun-Cheng; Kay, Lewis; Pawson, Tony; Forman-Kay, Julie
Citation:

Citation: Zwahlen, Catherine; Li, Shun-Cheng; Kay, Lewis; Pawson, Tony; Forman-Kay, Julie. "Multiple Modes of Peptide Recognition by the PTB Domain of the cell fate Determinant Numb"  EMBO J. 19, 1505-1515 (2000).

Assembly members:

Assembly members:
dNumb PTB, polymer, 171 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Drosophila melanogaster   Taxonomy ID: 32346   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts624
1H chemical shifts1079
15N chemical shifts161

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1dNumb PTB1

Entities:

Entity 1, dNumb PTB 171 residues - Formula weight is not available

1   GLYSERPROGLYILEPROASPARGVALPRO
2   GLUSERSERLYSPROHISGLNTRPGLNALA
3   ASPGLUGLUALAVALARGSERALATHRCYS
4   SERPHESERVALLYSTYRLEUGLYCYSVAL
5   GLUVALPHEGLUSERARGGLYMETGLNVAL
6   CYSGLUGLUALALEULYSVALLEUARGGLN
7   SERARGARGARGPROVALARGGLYLEULEU
8   HISVALSERGLYASPGLYLEUARGVALVAL
9   ASPASPGLUTHRLYSGLYLEUILEVALASP
10   GLNTHRILEGLULYSVALSERPHECYSALA
11   PROASPARGASNHISGLUARGGLYPHESER
12   TYRILECYSARGASPGLYTHRTHRARGARG
13   TRPMETCYSHISGLYPHELEUALACYSLYS
14   ASPSERGLYGLUARGLEUSERHISALAVAL
15   GLYCYSALAPHEALAVALCYSLEUGLUARG
16   LYSGLNARGARGTHRARGALAALAALASER
17   GLYPHESERASNMETSERPHEGLUASPPHE
18   PRO

Samples:

sample_1: dNumb PTB, [U-95% 13C; U-95% 15N], 1.2 mM

sample_2: dNumb PTB, [U-95% 15N], 1.2 mM

sample_3: dNumb PTB, [U-10% 13C; U-90% 15N], 1.0 mM

cond_1: pH: 6.5; temperature: 303 K; ionic strength: 0.15 M

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availablecond_1
1H-13C NOESYnot availablenot availablecond_1
HNCAnot availablenot availablecond_1
HNCACBnot availablenot availablecond_1
CBCACONNHnot availablenot availablecond_1
HBCBCACONNHnot availablenot availablecond_1
CCC-TOCSY-CONNHnot availablenot availablecond_1
HCC-TOCSY-CONNHnot availablenot availablecond_1
HCCH-TOCSYnot availablenot availablecond_1

Software:

NMRPipe - Processing

NMRView - Analysis

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz

Related Database Links:

BMRB 4263
PDB
GB AAA28730 AAF52776 AAK93152 AAN10693 ACL68752
REF NP_001260291 NP_523523 NP_723460 XP_001357245 XP_001964877
SP P16554
AlphaFold P16554

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks