BMRB Entry 4381

Title:
Letter to the Editor:Sequence-specific 1H, 13C, and 15N assignments for the third EH domain of Eps15 (EH3)
Deposition date:
1999-08-19
Original release date:
2000-03-20
Authors:
Enmon, Jennifer; de Beer, Tonny; Overduin, Michael
Citation:

Citation: Enmon, Jennifer; de Beer, Tonny; Overduin, Michael. "Letter to the Editor: Sequence-specific 1H, 13C, and 15N assignments for the third EH domain of Eps15 (EH3)"  J. Biomol. NMR 16, 81-82 (2000).

Assembly members:

Assembly members:
third EH domain of Eps15, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRSETA

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts445
15N chemical shifts92
1H chemical shifts713

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1EH31

Entities:

Entity 1, EH3 104 residues - Formula weight is not available

1   LYSARGLYSTHRTRPVALVALSERPROALA
2   GLULYSALALYSTYRASPGLUILEPHELEU
3   LYSTHRASPLYSASPMETASPGLYPHEVAL
4   SERGLYLEUGLUVALARGGLUILEPHELEU
5   LYSTHRGLYLEUPROSERTHRLEULEUALA
6   HISILETRPSERLEUCYSASPTHRLYSASP
7   CYSGLYLYSLEUSERLYSASPGLNPHEALA
8   LEUALAPHEHISLEUILESERGLNLYSLEU
9   ILELYSGLYILEASPPROPROHISVALLEU
10   THRPROGLUMETILEPROPROSERASPARG
11   ALASERLEUGLN

Samples:

sample_1: third EH domain of Eps15, [U-13C; U-15N], 1.0 mM; d-tris 10 mM; KCl 100 mM; NaN3 2 mM; APMSF 0.010 mM; d-DTT0.1 – 40 mM; D2O 10%; H2O 90%

sample_2: third EH domain of Eps15, [U-13C; U-15N], 1.0 mM; d-tris 10 mM; KCl 100 mM; NaN3 2 mM; APMSF 0.010 mM; d-DTT0.1 – 40 mM; D2O 99.9%

condition_1: pH: 7.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
CBCA(CO)NHnot availablenot availablecondition_1
HNCACBnot availablenot availablecondition_1
HCC-TOCSYnot availablenot availablecondition_1
CCC-TOCSYnot availablenot availablecondition_1
HCCH_TOCSYnot availablenot availablecondition_1
3D-15N-NOESYnot availablenot availablecondition_1
HNCOnot availablenot availablecondition_1
2D CB corelation with HD and HEnot availablenot availablecondition_1

Software:

NMRPipe - processing

PIPP - analysis

NMR spectrometers:

  • Varian Inova 500 MHz
  • Varian Inova 600 MHz

Related Database Links:

PDB
REF XP_006710509 XP_007976962 XP_009455980 XP_011539293 XP_011781647

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks