BMRB Entry 4335

Title:
NMR Assignments, Secondary Structure and Global Fold of Calerythrin, an EF-hand Calcium-binding Protein from Saccharopolyspora erythraea.
Deposition date:
1999-04-21
Original release date:
1999-12-22
Authors:
Aitio, Helena; Annila, Arto; Heikkinen, Sami; Thulin, Eva; Drakenberg, Torbjorn; Kilpelainen, Ilkka
Citation:

Citation: Aitio, Helena; Annila, Arto; Heikkinen, Sami; Thulin, Eva; Drakenberg, Torbjorn; Kilpelainen, Ilkka. "NMR Assignments, Secondary Structure and Global Fold of Calerythrin, an EF-hand Calcium-binding Protein from Saccharopolyspora erythraea."  Protein Sci. 8, 2580-2588 (1999).

Assembly members:

Assembly members:
calerythrin, polymer, 176 residues, Formula weight is not available
CALCIUM ION, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Streptomyces erythraeus   Taxonomy ID: 1836   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Saccharopolyspora erythraea

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts627
15N chemical shifts181
1H chemical shifts991

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1calerythrin1
2Ca2

Entities:

Entity 1, calerythrin 176 residues - Formula weight is not available

1   THRTHRALAILEALASERASPARGLEULYS
2   LYSARGPHEASPARGTRPASPPHEASPGLY
3   ASNGLYALALEUGLUARGALAASPPHEGLU
4   LYSGLUALAGLNHISILEALAGLUALAPHE
5   GLYLYSASPALAGLYALAALAGLUVALGLN
6   THRLEULYSASNALAPHEGLYGLYLEUPHE
7   ASPTYRLEUALALYSGLUALAGLYVALGLY
8   SERASPGLYSERLEUTHRGLUGLUGLNPHE
9   ILEARGVALTHRGLUASNLEUILEPHEGLU
10   GLNGLYGLUALASERPHEASNARGVALLEU
11   GLYPROVALVALLYSGLYILEVALGLYMET
12   CYSASPLYSASNALAASPGLYGLNILEASN
13   ALAASPGLUPHEALAALATRPLEUTHRALA
14   LEUGLYMETSERLYSALAGLUALAALAGLU
15   ALAPHEASNGLNVALASPTHRASNGLYASN
16   GLYGLULEUSERLEUASPGLULEULEUTHR
17   ALAVALARGASPPHEHISPHEGLYARGLEU
18   ASPVALGLULEULEUGLY

Entity 2, Ca - Ca - 40.078 Da.

1   CA

Samples:

sample_one: calerythrin, [U-13C; U-15N], 1.0 mM; CaCl2 4 mM; DTT 10 mM; H2O 97%; D2O 3%

sample_conditions: pH: 6.0 na; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
HNCACBsample_onenot availablesample_conditions
HN(CO)CACBsample_onenot availablesample_conditions
CC(CO)NHsample_onenot availablesample_conditions
HNCOsample_onenot availablesample_conditions
HCCH-TOCSYsample_onenot availablesample_conditions
[15N]-edited NOESYsample_onenot availablesample_conditions
[15N,15N]-edited NOESYsample_onenot availablesample_conditions
[15N,13C]-edited NOESYsample_onenot availablesample_conditions
[15N]-edited TOCSYsample_onenot availablesample_conditions

Software:

FELIX v97.0 -

NMR spectrometers:

  • Varian Unity 500 MHz
  • Varian Unity 600 MHz

Related Database Links:

PDB
EMBL CAM02479
GB AAA26481 EQD82838
PRF 1311341A
REF WP_009945618
SP P06495
AlphaFold P06495

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks