BMRB Entry 4331

Title:
Backbone 1H, 13C, and 15N Resonance Assignments of Streptomyces Subtilisin Inhibitor
Deposition date:
1999-04-12
Original release date:
1999-05-24
Authors:
Sasakawa, Hiroaki; Tamura, Atsuo; Taguchi, Seiichi; Akasaka, Kazuyuki; Miyake, Yoko; Kainosho, Masatsune
Citation:

Citation: Sasakawa, Hiroaki; Tamura, Atsuo; Taguchi, Seiichi; Akasaka, Kazuyuki; Miyake, Yoko; Kainosho, Masatsune. "Backbone 1H, 13C, and 15N Resonance Assignments of Streptomyces Subtilisin Inhibitor"  J. Biomol. NMR ., .-. (1999).

Assembly members:

Assembly members:
Streptomyces subtilisin inhibitor, polymer, 113 residues, 11500 Da.

Natural source:

Natural source:   Common Name: S. albogriseolus   Taxonomy ID: 1887   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Streptomyces albogriseolus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts198
15N chemical shifts98
1H chemical shifts98

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SSI subunit 11
2SSI subunit 21

Entities:

Entity 1, SSI subunit 1 113 residues - 11500 Da.

1   GLYALAPROSERALALEUTYRALAPROSER
2   ALALEUVALLEUTHRVALGLYLYSGLYVAL
3   SERALATHRTHRALAALAPROGLUARGALA
4   VALTHRLEUTHRCYSALAPROGLYPROSER
5   GLYTHRHISPROALAALAGLYSERALACYS
6   ALAASPLEUALAALAVALGLYGLYASPLEU
7   ASNALALEUTHRARGGLYGLUASPVALMET
8   CYSPROMETVALTYRASPPROVALLEULEU
9   THRVALASPGLYVALTRPGLNGLYLYSARG
10   VALSERTYRGLUARGVALPHESERASNGLU
11   CYSGLUMETASNALAHISGLYSERSERVAL
12   PHEALAPHE

Samples:

sample_one: Streptomyces subtilisin inhibitor, [U-13C; U-15N], mM; phosphate 25 mM

sample_conditions: pH: 6.3 na; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCOnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 750 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks