BMRB Entry 4323

Title:
1H, 13C, 15N NMR backbone assignments of 37 kDa surface antigen OspC from Borrelia burgdorferi
Deposition date:
1999-03-22
Original release date:
2000-12-15
Authors:
Huang, Xiaolin; Link, Karl; Koide, Akiko; Dunn, J.; Luft, B.; Koide, Shohei
Citation:

Citation: Huang, Xiaolin; Link, Karl; Koide, Akiko; Dunn, J.; Luft, B.; Koide, Shohei. "1H, 13C, 15N NMR backbone assignments of 37 kDa surface antigen OspC from Borrelia burgdorferi"  J. Biomol. NMR 14, 283-284 (1999).

Assembly members:

Assembly members:
outer surface protein C, polymer, 174 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: spirochete   Taxonomy ID: 139   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Borrelia burgdorferi

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24-OspC

Data sets:
Data typeCount
13C chemical shifts495
15N chemical shifts168
1H chemical shifts168

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1OspC monomer 11
2OspC monomer 21

Entities:

Entity 1, OspC monomer 1 174 residues - Formula weight is not available

1   METLYSGLYPROASNLEUTHRGLUILESER
2   LYSLYSILETHRASPSERASNALAVALLEU
3   LEUALAVALLYSGLUVALGLUALALEULEU
4   SERSERILEASPGLUILEALAALALYSALA
5   ILEGLYLYSLYSILEHISGLNASNASNGLY
6   LEUASPTHRGLUASNASNHISASNGLYSER
7   LEULEUALAGLYALATYRALAILESERTHR
8   LEUILELYSGLNLYSLEUASPGLYLEULYS
9   ASNGLUGLYLEULYSGLULYSILEASPALA
10   ALALYSLYSCYSSERGLUTHRPHETHRASN
11   LYSLEULYSGLULYSHISTHRASPLEUGLY
12   LYSGLUGLYVALTHRASPALAASPALALYS
13   GLUALAILELEULYSTHRASNGLYTHRLYS
14   THRLYSGLYALAGLUGLULEUGLYLYSLEU
15   PHEGLUSERVALGLUVALLEUSERLYSALA
16   ALALYSGLUMETLEUALAASNSERVALLYS
17   GLULEUTHRSERPROVALVALALAGLUSER
18   PROLYSLYSPRO

Samples:

sample_one: outer surface protein C, [U-99% 13C; U-98% 15N; U-95% 2H], 1.0 mM; sodium phosphate 20 mM; sodium chloride 100 mM; EDTA 0.10 mM; DTT 1.0 mM

sample_conditions_one: ionic strength: 120 mM; pH: 6.0; pressure: 1.0 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

PDB
DBJ BAA08457
EMBL CAA49306 CAA52001 CAA59254 CAG44432 CAG44433
GB AAA16058 AAB06569 AAB36995 AAB81889 AAB81894
PRF 2003376B
REF NP_047005 WP_010890595
SP Q07337
AlphaFold Q07337

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks