BMRB Entry 4316

Title:
1H, 13C and 15N Chemical Shift Assignements for Subunit c of the ATP Synthase from Propionigenium modestum
Deposition date:
1999-03-11
Original release date:
1999-05-27
Authors:
Matthey, Ulrich; Kaim, Georg; Braun, Daniel; Wuthrich, Kurt; Dimroth, Peter
Citation:

Citation: Matthey, Ulrich; Kaim, Georg; Braun, Daniel; Wuthrich, Kurt; Dimroth, Peter. "NMR Studies of Subunit C of the ATP Synthase from Propionigenium modestum in Dodecylsulfate Micelles"  Eur. J. Biochem. 261, 459-467 (1999).

Assembly members:

Assembly members:
ATP synthase subunit c, polymer, 89 residues, 8731 Da.

Natural source:

Natural source:   Common Name: P. modestum   Taxonomy ID: 2333   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Propionigenium modestum

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: plasmid

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts269
15N chemical shifts85
1H chemical shifts617

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ATP synthase subunit c1

Entities:

Entity 1, ATP synthase subunit c 89 residues - 8731 Da.

1   METASPMETVALLEUALALYSTHRVALVAL
2   LEUALAALASERALAVALGLYALAGLYALA
3   ALAMETILEALAGLYILEGLYPROGLYVAL
4   GLYGLNGLYTYRALAALAGLYLYSALAVAL
5   GLUSERVALALAARGGLNPROGLUALALYS
6   GLYASPILEILESERTHRMETVALLEUGLY
7   GLNALAILEALAGLUSERTHRGLYILETYR
8   SERLEUVALILEALALEUILELEULEUTYR
9   ALAASNPROPHEVALGLYLEULEUGLY

Samples:

sample_one: ATP synthase subunit c, [U-15N], 1.5 mM; SDS deuterated 300 mM

sample_two: ATP synthase subunit c, [U-15N; U-13C], 1.9 mM; SDS deuterated 300 mM

Pmc_set1: ionic strength: .42 na; pH: 5.8 na; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D [15N,1H]-HSQCnot availablenot availablenot available
2D ct-[13C,1H]-HSQCnot availablenot availablenot available
3D HNCAnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D 15N-res. [1H,1H]-NOESY (mixing time: 60 ms)not availablenot availablenot available
3D 15N-res. [1H,1H]-TOCSY (mixing time: 60 ms)not availablenot availablenot available
3D HCCH-COSYnot availablenot availablenot available
3D HCCH-TOCSY (mixing time: 17.2 ms)not availablenot availablenot available
3D 13C-res. [1H,1H]-NOESY (mixing time: 50 ms)not availablenot availablenot available
2D [1H, 1H]-TOCSYnot availablenot availablenot available
2D [1H, 1H]-NOESYnot availablenot availablenot available
3D 13C/13C-res. [1H,1H]-NOESY (mixing time: 60 ms)not availablenot availablenot available
2D 15N{1H}-NOEnot availablenot availablenot available
The 13C/13C-res. [1H, 1H]-NOESY were recordednot availablenot availablenot available
to verify some resonance assignments.not availablenot availablenot available

Software:

PROSA - data processing

XEASY - spectral analyses

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 600 MHz
  • Bruker DRX 750 MHz
  • Varian Unity+ 750 MHz

Related Database Links:

EMBL CAA37840 CAA37912 CAA41369 CAA46895
GB AAB22156
SP P21905
AlphaFold P21905

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks