BMRB Entry 4187
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4187
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NMR-STAR v3 text file.
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Citation: Tonelli, M.; Ragg, E.; Bianucci, A.; Lesiak, K.; James, T.. "Nuclear Magnetic Resonance Structure of d(GCATATGATAG).d(CTATCATATGC): A
Consensus Sequence for Promoters Recognized by Sigma-K RNA Polymerase" Biochemistry 37, 11745-11761 (1998).
Assembly members:
Promoter strand 1, polymer, 11 residues, Formula weight is not available
Promoter strand 2, polymer, 11 residues, Formula weight is not available
Natural source: Common Name: Bacillus subtilis Taxonomy ID: 1423 Superkingdom: Eubacteria Kingdom: not available Genus/species: Bacillus subtilis
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Promoter strand 1: GCATATGATAG
Promoter strand 2: CTATCATATGC
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 204 |
| coupling constants | 48 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DNA 1 | 1 |
| 2 | DNA 2 | 2 |
Entities:
Entity 1, DNA 1 11 residues - Formula weight is not available
| 1 | DG | DC | DA | DT | DA | DT | DG | DA | DT | DA | ||||
| 2 | DG |
Entity 2, DNA 2 11 residues - Formula weight is not available
| 1 | DC | DT | DA | DT | DC | DA | DT | DA | DT | DG | ||||
| 2 | DC |
Samples:
sample_one: Promoter strand 1 2.5 mM; Promoter strand 2 2.5 mM
sample_conditions_one: ionic strength: 0.5 M; pH: 7.0; pressure: 1 atm; temperature: 298.0 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY | sample_one | not available | sample_conditions_one |
| COSY | sample_one | not available | sample_conditions_one |
Software:
Striker - Spectral processing
SPARKY - Spectral assignment
AMBER - Structure calculation via molecular dynamic simulations
DNAminiCarlo - Structure calculation via Monte Carlo calculations
NMR spectrometers:
- BRUKER AMX 600 MHz
Related Database Links:
| PDB |