BMRB Entry 4145

Title:
Assignment of 1H and 15N Resonances of Murine Tec SH3 Domain
Deposition date:
1998-05-27
Original release date:
1999-02-03
Authors:
Pursglove, Sharon; Mulhern, Terrence; Booker, Grant
Citation:

Citation: Pursglove, Sharon; Mulhern, Terrence; Hinds, Mark; Norton, Raymond; Booker, Grant. "Assignment of 1H and 15N Resonances of Murine Tec SH3 Domain"  J. Biomol. NMR 12, 461-462 (1998).

Assembly members:

Assembly members:
Tec src homology 3 (SH3) domain, polymer, 67 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Tec src homology 3 (SH3) domain: GSEIVVAMYDFQATEAHDLR LERGQEYIILEKNDLHWWRA RDKYGSEGYIPSNYVTGKKS NNLDQYD

Data sets:
Data typeCount
15N chemical shifts76
1H chemical shifts459

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Tec_SH31

Entities:

Entity 1, Tec_SH3 67 residues - Formula weight is not available

1   GLYSERGLUILEVALVALALAMETTYRASP
2   PHEGLNALATHRGLUALAHISASPLEUARG
3   LEUGLUARGGLYGLNGLUTYRILEILELEU
4   GLULYSASNASPLEUHISTRPTRPARGALA
5   ARGASPLYSTYRGLYSERGLUGLYTYRILE
6   PROSERASNTYRVALTHRGLYLYSLYSSER
7   ASNASNLEUASPGLNTYRASP

Samples:

sample_one: Tec src homology 3 (SH3) domain, [U-15N], 1.25 – 2 mM

sample_conditions_one: pH: 6.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N TOCSYsample_onenot availablesample_conditions_one
1H-15N NOESYsample_onenot availablesample_conditions_one
H20 TOCSY, NOESY, COSYsample_onenot availablesample_conditions_one
D20 TOCSY, NOESY, COSYsample_onenot availablesample_conditions_one

Software:

XEASY -

NMR spectrometers:

  • Varian Inova 600 MHz
  • Bruker AMX 500 MHz
  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAE23957 BAE32599 BAE40357 BAE41897
EMBL CAA39196
GB AAA13515 AAD43402 AAD43405 AAD43406 AAD43407
REF NP_001106931 NP_001106932 NP_001106935 NP_038717 NP_445884
SP P24604
AlphaFold P24604

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks