BMRB Entry 4109

Title:
Sequence Specific 1H, 13C and 15N Resonance Assignment of Rat CD2 Domain 1
Deposition date:
1998-02-18
Original release date:
1999-02-02
Authors:
Chen, Ho; Pfuhl, Mark; Davis, Ben; Driscoll, Paul
Citation:

Citation: Chen, Ho; Pfuhl, Mark; Davis, Ben; Driscoll, Paul. "Sequence Specific 1H, 13C and 15N Resonance Assignment of Rat CD2 Domain 1"  J. Biomol. NMR 12, 457-458 (1998).

Assembly members:

Assembly members:
rat CD2 domain 1, polymer, 100 residues, 12000 Da.

Natural source:

Natural source:   Common Name: rat   Taxonomy ID: 10117   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus rattus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data typeCount
13C chemical shifts1055
15N chemical shifts302
1H chemical shifts1921

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CD2d11

Entities:

Entity 1, CD2d1 100 residues - 12000 Da.

1   METARGASPSERGLYTHRVALTRPGLYALA
2   LEUGLYHISGLYILEASNLEUASNILEPRO
3   ASNPHEGLNMETTHRASPASPILEASPGLU
4   VALARGTRPGLUARGGLYSERTHRLEUVAL
5   ALAGLUPHELYSARGLYSMETLYSPROPHE
6   LEULYSSERGLYALAPHEGLUILELEUALA
7   ASNGLYASPLEULYSILELYSASNLEUTHR
8   ARGASPASPSERGLYTHRTYRASNVALTHR
9   VALTYRSERTHRASNGLYTHRARGILELEU
10   ASNLYSALALEUASPLEUARGILELEUGLU

Samples:

sample_one: rat CD2 domain 1, [U96%-13C;U96%-15N], 1.2 mM; phosphate buffer 20 mM

sample_conditions_one: pH: 3.0; temperature: 298 K

sample_conditions_two: pH: 5.0; temperature: 298 K

sample_conditions_three: pH: 7.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HN(CO)CACBnot availablenot availablenot available
CBCACOCAHAnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
HCCH-COSYnot availablenot availablenot available
HC(C)COnot availablenot availablenot available
1H-15N HSQCnot availablenot availablenot available
1H-13C CT-HSQCnot availablenot availablenot available

Software:

NMRPipe - processing of spectra

XEASY - Spectral analysis

NMR spectrometers:

  • Varian UnityPlus 600 MHz

Related Database Links:

PDB
EMBL CAA28757
GB AAH88164 ADI96088 EDL85525
REF NP_036962
SP P08921
AlphaFold P08921

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks