BMRB Entry 36751

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR36751
MolProbity Validation Chart

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NMR-STAR v3 text file.
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Title:
effector of a plant pathogen
Deposition date:
2025-04-02
Original release date:
2026-06-06
Authors:
Ohki, S.; Mori, M.
Citation:

Citation: Ohki, Shinya; Takahara, Hiroyuki; Imamura, Tomohiro; Sakane, Kosei; Bai, Asihan; Sasaki, Kazunori; Nishiuchi, Takumi; Mori, Masashi. "Necrosis-Suppressing Effector Protein ChEC88 Adopts a Novel Structural Motif Conserved Among Genus-Spanning Hemibiotrophic Phytopathogens."  Plants (Basel). 14, .-. (2025).
PubMed: 40872185

Assembly members:

Assembly members:
EC88, polymer, 78 residues, 8209.192 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 80884   Superkingdom: not available   Kingdom: Fungi   Genus/species: Colletotrichum higginsianum

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET32b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
EC88: QLPVSNEVCPAGLRTTCPAG TDGVSRCSILNGQNLCVIDC EAQSTCRTQCKQQGHINGFC TIGDNPCICSDIDPGFNN

Data sets:
Data typeCount
13C chemical shifts211
15N chemical shifts73
1H chemical shifts463

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 78 residues - 8209.192 Da.

1   GLNLEUPROVALSERASNGLUVALCYSPRO
2   ALAGLYLEUARGTHRTHRCYSPROALAGLY
3   THRASPGLYVALSERARGCYSSERILELEU
4   ASNGLYGLNASNLEUCYSVALILEASPCYS
5   GLUALAGLNSERTHRCYSARGTHRGLNCYS
6   LYSGLNGLNGLYHISILEASNGLYPHECYS
7   THRILEGLYASPASNPROCYSILECYSSER
8   ASPILEASPPROGLYPHEASNASN

Samples:

sample_1: ChEC88, [U-13C; U-15N], 0.6 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 50 mM; pH: 6.5 Not defined; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1

Software:

CYANA v2, Guntert, Mumenthaler and Wuthrich - structure calculation

X-PLOR NIH v3.1, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMRFAM-SPARKY, Lee W, Tonelli M, Markley JL - chemical shift assignment

NMRFAM-SPARKY, Lee W, Tonelli M, Markley JL - peak picking

NMR spectrometers:

  • Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks