BMRB Entry 36684

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR36684
MolProbity Validation Chart

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Title:
D-Amino Acid Substituted Antimicrobial Peptides Derived from Tilapia piscidin 4
Deposition date:
2024-07-08
Original release date:
2026-02-06
Authors:
Huang, Y.; Chang, C.
Citation:

Citation: Huang, Y.; Chang, C.. "D-Amino Acid Substituted Antimicrobial Peptides Derived from Tilapia piscidin 4"  .

Assembly members:

Assembly members:
Tilapia piscidin 4 alpha, polymer, 25 residues, 3089.948 Da.

Natural source:

Natural source:   Common Name: Nile tilapia   Taxonomy ID: 8128   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Oreochromis niloticus

Experimental source:

Experimental source:   Production method: chemical synthesis   Host organism: unidentified

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Tilapia piscidin 4 alpha: FLXKLXKAXFSXPKXLKXLL XRRXR

Data sets:
Data typeCount
1H chemical shifts202

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 25 residues - 3089.948 Da.

1   PHELEUDLYLYSLEUDLELYSALADLEPHE
2   SERDALPROLYSDALLEULYSDARLEULEU
3   DARARGARGDARARG

Samples:

sample_1: TP4a 2 mM; PBS 50 % v/v; D2O, [U-2H], 10 % v/v; TFE-d3, [U-2H], 40 % v/v

sample_conditions_1: ionic strength: 150 mM; pH: 6.92; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

NMRFAM-SPARKY, Woonghee Lee and Poky Team Members (CU Denver) - chemical shift assignment

CYANA v3.98.13, Guntert, Mumenthaler and Wuthrich - structure calculation

CYANA, Guntert, Mumenthaler and Wuthrich - refinement

NMRFAM-SPARKY, Woonghee Lee and Poky Team Members (CU Denver) - peak picking

NMR spectrometers:

  • Bruker AVANCE 800 MHz
  • Bruker AVANCE 800 MHz