BMRB Entry 36683

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR36683
MolProbity Validation Chart

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NMR-STAR v3 text file.
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Title:
Tilapia Piscidin-TP2-5
Deposition date:
2024-07-08
Original release date:
2026-02-06
Authors:
Huang, Y.; Chang, C.
Citation:

Citation: Huang, Y.; Chang, C.. "Tilapia Piscidin-TP2-5"  .

Assembly members:

Assembly members:
LYS-LYS-CYS-ILE-ALA-LYS-ALA-ILE-LEU-LYS-LYS-ALA-LYS-LYS-LEU-LEU-LYS-LYS-LEU-VAL-ASN-PRO, polymer, 22 residues, 2486.305 Da.

Natural source:

Natural source:   Common Name: Nile tilapia   Taxonomy ID: 8128   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Oreochromis niloticus

Experimental source:

Experimental source:   Production method: chemical synthesis   Host organism: unidentified

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LYS-LYS-CYS-ILE-ALA-LYS-ALA-ILE-LEU-LYS-LYS-ALA-LYS-LYS-LEU-LEU-LYS-LYS-LEU-VAL-ASN-PRO: KKCIAKAILKKAKKLLKKLV NP

Data sets:
Data typeCount
1H chemical shifts179

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 22 residues - 2486.305 Da.

1   LYSLYSCYSILEALALYSALAILELEULYS
2   LYSALALYSLYSLEULEULYSLYSLEUVAL
3   ASNPRO

Samples:

sample_1: TP2-5 2 M; PBS 60 % v/v; D2O, [U-2H], 10 % v/v; TFE-d3, [U-2H], 30 % v/v

sample_conditions_1: ionic strength: 150 mM; pH: 3; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H COSYsample_1anisotropicsample_conditions_1
2D 1H-1H TOCSYsample_1anisotropicsample_conditions_1
2D 1H-1H NOESYsample_1anisotropicsample_conditions_1

Software:

NMRFAM-SPARKY, Woonghee Lee and Poky Team Members (CU Denver) - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

CYANA, Guntert, Mumenthaler and Wuthrich - refinement

NMRFAM-SPARKY, Woonghee Lee and Poky Team Members (CU Denver) - peak picking

NMR spectrometers:

  • Bruker AVANCE III 600 MHz