BMRB Entry 36447

Title:
NMR structure of the zeta-subunit of the F1F0-ATPase from Sinorhizobium meliloti
Deposition date:
2021-10-01
Original release date:
2022-03-22
Authors:
Yang, L.; Mendoza-Hoffmann, F.; Buratto, D.
Citation:

Citation: Yang, L.; Mendoza-Hoffmann, F.; Buratto, D.. "NMR structure of the zeta-subunit of the F1F0-ATPase from Sinorhizobium meliloti"  .

Assembly members:

Assembly members:
zeta-subunit, polymer, 104 residues, 11616.140 Da.

Natural source:

Natural source:   Common Name: Ensifer meliloti, Sinorhizobium meliloti   Taxonomy ID: 266834   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Sinorhizobium meliloti

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Data typeCount
13C chemical shifts364
15N chemical shifts96
1H chemical shifts559

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 104 residues - 11616.140 Da.

1   METTHRTHRMETGLNASPARGGLULYSALA
2   PHEGLUALALYSPHEALALEUASPGLUGLU
3   LEUARGPHELYSALATHRALAARGARGASN
4   LYSLEULEUGLYLEUTRPALAALAGLYLEU
5   LEUALALYSSERASPPROGLUALATYRALA
6   SERGLUILEVALALAALAASPPHEGLUGLU
7   ALAGLYHISGLUASPVALVALARGLYSILE
8   LYSTHRASPPHEASPALAALAGLYVALALA
9   ILESERGLUASPASPILEARGVALARGMET
10   ILEGLULEULEUSERGLUALAVALALAGLN
11   LEUGLNLYSASN

Samples:

sample_1: zeta-subunit, [U-13C; U-15N], 1.0 mM; PBS 25 mM; NaCl 50 mM; NaN3 4.5 mM; H2O 95%; D2O, [U-2H], 5%

sample_conditions_1: ionic strength: 75 mM; pH: 6.8; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
5D APSY CBCACONHsample_1isotropicsample_conditions_1
4D APSY HACANHsample_1isotropicsample_conditions_1
5D APSY HACACONHsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

GROMACS, John Kerrigan - refinement

TopSpin, Bruker Biospin - collection

UNIO, Torsten Herrmann - chemical shift assignment, structure calculation

NMR spectrometers:

  • Bruker AVANCE III HD 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks