BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 36417

Title: Prion Derived Tetrapeptide Stabilizes Thermolabile Insulin via Conformational Trapping

Deposition date: 2021-04-11 Original release date: 2021-05-11

Authors: Banerjee, N.

Citation: Banerjee, N.. "Prion Derived Tetrapeptide Stabilizes Thermolabile Insulin via Conformational Trapping"  .

Assembly members:
entity_1, polymer, 21 residues, 2339.645 Da.
entity_2, polymer, 30 residues, 3403.927 Da.
entity_3, polymer, 4 residues, 600.687 Da.

Natural source:   Common Name: Bovine   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: GIVEQCCASVCSLYQLENYC N
entity_2: FVNQHLCGSHLVEALYLVCG ERGFFYTPKA
entity_3: VYYR

Data sets:
Data typeCount
1H chemical shifts316

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11
2unit_22
3unit_33

Entities:

Entity 1, unit_1 21 residues - 2339.645 Da.

1   GLYILEVALGLUGLNCYSCYSALASERVAL
2   CYSSERLEUTYRGLNLEUGLUASNTYRCYS
3   ASN

Entity 2, unit_2 30 residues - 3403.927 Da.

1   PHEVALASNGLNHISLEUCYSGLYSERHIS
2   LEUVALGLUALALEUTYRLEUVALCYSGLY
3   GLUARGGLYPHEPHETYRTHRPROLYSALA

Entity 3, unit_3 4 residues - 600.687 Da.

1   VALTYRTYRARG

Samples:

sample_1: Bovine Insulin, Proton, 1 mM; Peptide, Proton-15N, 13C, 1 mM; Citrate-Phoshate 1 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 1 mM; pH: 2.6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1D 1Hsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRFAM-SPARKY, Lee W, Tonelli M, Markley JL - structure calculation

TopSpin, Bruker Biospin - chemical shift assignment

TopSpin v3.1, Bruker Biospin - refinement

NMR spectrometers:

  • Bruker AVANCE III 500 MHz
  • Bruker AVANCE III 700 MHz