BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 36294

Title: membrane-bound Bax helix2-helix5 domain

Deposition date: 2019-11-07 Original release date: 2021-05-11

Authors: Bo, O.; Fujiao, L.

Citation: Bo, O.; Fujiao, L.. "membrane-bound Bax helix2-helix5 domain"  .

Assembly members:
Apoptosis regulator BAX, polymer, 81 residues, 8990.265 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):
Apoptosis regulator BAX: GPGSMDASTKKLSESLKRIG DELDSNMELQRMIAAVDTDS PREVFFRVAADMFSDGNFNW GRVVALFYFASKLVLKALST K

Data sets:
Data typeCount
13C chemical shifts233
15N chemical shifts77
1H chemical shifts77

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_1, 11
2entity_1, 21

Entities:

Entity 1, entity_1, 1 81 residues - 8990.265 Da.

1   GLYPROGLYSERMETASPALASERTHRLYS
2   LYSLEUSERGLUSERLEULYSARGILEGLY
3   ASPGLULEUASPSERASNMETGLULEUGLN
4   ARGMETILEALAALAVALASPTHRASPSER
5   PROARGGLUVALPHEPHEARGVALALAALA
6   ASPMETPHESERASPGLYASNPHEASNTRP
7   GLYARGVALVALALALEUPHETYRPHEALA
8   SERLYSLEUVALLEULYSALALEUSERTHR
9   LYS

Samples:

sample_1: human Bax core domain, [U-98% 15N; U-98% 13C; U-98% 2H], 0.5 mM; DHPC 140 mM; DMPC 100 mM; MES 10 mM; sodium chloride 100 mM; H2O 90%; D2O, [U-2H], 10%

sample_2: Bax a_2_a_5, [U-98% 15N; U-98% 13C], 0.6 mM; DHPC, [U-2H], 160 mM; DMPC, [U-2H], 100 mM; MES 10 mM; sodium chloride 100 mM; H2O 90%; D2O, [U-2H], 10%

sample_3: Bax a_2_a_5, U-98% 15N;U-2H, 0.4 mM; DHPC, [U-2H], 200 mM; DMPC, [U-2H], 120 mM; MES 10 mM; sodium chloride 100 mM; H2O 90%; D2O, [U-2H], 10%

sample_4: Bax a_2_a_5, [U-98% 15N; U-2H], 0.6 mM; DHPC, [U-2H], 160 mM; DMPC 100 mM; MES 10 mM; sodium chloride 100 mM; H2O 90%; D2O, [U-2H], 10%

sample_5: Bax a_2_a_5, [U-98% 15N; U-15% 13C], 0.4 mM; DHPC 140 mM; DMPC 80 mM; MES 10 mM; sodium chloride 100 mM; H2O 90%; D2O, [U-2H], 10%

sample_6: Bax a_2_a_5, [U-98% 13C], 0.4 mM; DHPC, [U-2H], 200 mM; DMPC, [U-2H], 120 mM; MES 10 mM; sodium chloride 100 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 100 mM; pH: 6.0; pressure: 1 atm; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
2D TROSY-HSQCsample_1isotropicsample_conditions_1
TROSY-HNCAsample_1isotropicsample_conditions_1
TROSY-HNCOCAsample_1isotropicsample_conditions_1
TROSY-HNCACOsample_1isotropicsample_conditions_1
TROSY-HNCOsample_1isotropicsample_conditions_1
3D 15N NOE-TROSY-HSQCsample_1isotropicsample_conditions_1
3D 15N NOE-TROSY-HSQCsample_2isotropicsample_conditions_1
3D 13C NOE-HSQCsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D 15N NOE-TROSY-HSQCsample_3isotropicsample_conditions_1
2D TROSY-HSQCsample_3isotropicsample_conditions_1
3D 15N NOE-TROSY-HSQCsample_4isotropicsample_conditions_1
2D TROSY-HSQCsample_4isotropicsample_conditions_1
2D 1H-13C HSQCsample_5isotropicsample_conditions_1
TROSY-HNCACBsample_1isotropicsample_conditions_1

Software:

CcpNmr Analysis, CCPN - peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TALOS, Cornilescu, Delaglio and Bax - data analysis

TopSpin, Bruker Biospin - collection

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

XEASY, Bartels et al. - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE 600 MHz
  • Bruker AVANCE 900 MHz
  • Agilent DD2 800 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts