BMRB Entry 36157

Title:
Nukacin ISK-1 in active state
Deposition date:
2018-01-19
Original release date:
2019-06-05
Authors:
Kohda, D.; Fujinami, D.
Citation:

Citation: Fujinami, Daisuke; Mahin, Abdullah-Al; Elsayed, Khaled M; Islam, Mohammad R; Nagao, Jun-Ichi; Roy, Urmi; Momin, Sabrina; Zendo, Takeshi; Kohda, Daisuke; Sonomoto, Kenji. "The lantibiotic nukacin ISK-1 exists in an equilibrium between active and inactive lipid-II binding states."  Commun. Biol. 1, 150-150 (2018).
PubMed: 30272026

Assembly members:

Assembly members:
Lantibiotic nukacin, polymer, 27 residues, 2970.535 Da.

Natural source:

Natural source:   Common Name: Staphylococcus warneri   Taxonomy ID: 1292   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus warneri

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Lantibiotic nukacin: KKKSGVIPXVXHDCHMNXFQ FVFXCCS

Data sets:
Data typeCount
1H chemical shifts174

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 27 residues - 2970.535 Da.

1   LYSLYSLYSSERGLYVALILEPRODBBVAL
2   DALHISASPCYSHISMETASNDALPHEGLN
3   PHEVALPHEDBUCYSCYSSER

Samples:

sample_1: dodecylphosphocholine, [U-2H], 34 mM; glycine 10 mM; nukacin ISK-1 13 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 0 mM; pH: 3.5; pressure: 1 atm; temperature: 328 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert P. - refinement

NMR spectrometers:

  • Bruker Avance 700 MHz